Molecular characterization of angiogenic properties of human oral squamous cell carcinoma cells

• Published in: Oral oncology Vol. 38; no. 7; pp. 699 - 705
• Main Authors: Chen, Yi-Ju, Jin, Ying-Tai, Shieh, Dar-Bin, Tsai, Sen-Tien, Wu, Li-Wha
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2002; Elsevier
• Subjects: Angiogenesis; Biological and medical sciences; Carcinoma, Squamous Cell - metabolism; Cell Division; Cell Movement; Endothelial Growth Factors - metabolism; Endothelium, Vascular - cytology; Gene Expression - genetics; Genes, p53 - genetics; Humans; Intercellular Signaling Peptides and Proteins - metabolism; Keratinocytes; Keratinocytes - metabolism; Lymphokines - metabolism; Mdm2; Medical sciences; Mouth Neoplasms - metabolism; Neoplasm Proteins - metabolism; Neovascularization, Pathologic - etiology; Nuclear Proteins; Oral cancer; Otorhinolaryngology. Stomatology; p53; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Proto-Oncogene Proteins c-mdm2; Squamous cell carcinoma; Tumor Cells, Cultured; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors; VEGF; Angiogenesis; Squamous cell carcinoma; Oral cancer; VEGF; Mdm2; Keratinocytes; p53; Cell culture; Stomatology; Pathogenesis; Cytokine; Characteristic; Malignant tumor; Experimental study; Mechanism; Oral cavity; Specificity; Vascular endothelium growth factor; TP53 Gene; Polypeptide; Blotting assay; Genetics; Keratinocyte; Oral cavity disease; Molecular biology; ELISA assay; Growth factor
• ISSN: 1368-8375; 1879-0593
• DOI: 10.1016/S1368-8375(02)00004-0

Little is known about the specificity of angiogenic properties of oral cancer cells and the possible mechanisms. Stimulatory effects on proliferation and migration of human umbilical vein endothelial cells (HUVEC) characterized the angiogenic properties of oral cancer cells but not normal oral keratinocytes (NOK). ELISA found the presence of vascular endothelial growth factors (VEGF) both in the tested oral cancer cells and NOK. Attenuation of the proangiogenic effects by neutralizing VEGF antibodies suggests VEGF play a key role in the acquisition of the angiogenic phenotype in oral cancer cells. Western blotting of p53 and murine double mutant 2 (Mdm2) together with p53 DNA sequencing analysis indicate that p53 function loss by mutation or overexpression of Mdm2 occurred in all tested oral cancer cells regardless of their etiology. In summary, the angiogenic property of oral cancer cells is mediated by many factors in addition to VEGF and the functional status of p53.