Silole nanocrystals as novel biolabels

• Published in: Journal of immunological methods Vol. 295; no. 1; pp. 111 - 118
• Main Authors: Pui-yee Chan, Cangel, Haeussler, Matthias, Zhong Tang, Ben, Dong, Yongqiang, Sin, King-keung, Mak, Wing-cheung, Trau, Dieter, Seydack, Matthias, Renneberg, Reinhard
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2004; Elsevier
• Subjects: Aggregation-induced emission (AIE); Biolabel; Biological and medical sciences; Crystallization; Fluorescence immunoassay (FIA); Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunoassay - methods; Layer-by-layer (LbL) technique; Molecular immunology; Nanotechnology; Pilot Projects; Silole; Spectrophotometry; Techniques; PBS; FDA; Layer-by-layer (LbL) technique; SDS; HPC-SL; Ph 2Si(CPh) 4; HPS; Biolabel; LED; Silole; BSA; PSS; FITC; THF; PAH; Aggregation-induced emission (AIE); LbL; Gt α M IgG; M IgG; SEM; AIE; F/P ratio; Fluorescence immunoassay (FIA); FIA; Aggregation; Immunological method
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2004.09.016

A novel class of biofunctional silole nanocrystals with the potential to create highly sensitive immunoassay was firstly demonstrated. Biolabels were constructed by encapsulating nanocrystalline hexaphenylsilole [Ph 2Si(CPh) 4; HPS] within ultrathin polyelectrolyte layers via the layer-by-layer (LbL) technique that provided an “interface” for the attachment of antibodies. A high ratio of fluorescent dyes to biomolecules (F/P ratio; 2.4×10 3) was achieved without self-quenching problem. The aggregation-induced emission (AIE) feature offered silole biolabels the sensitivity 40- to 140-fold higher than that of a start-of-the-art immunoassay using directly fluorescent-labeled antibodies.