Molecular Identification of `Candidatus Mycoplasma haemovis' in Sheep with Hemolytic Anemia

• Published in: Journal of Veterinary Medical Science Vol. 73; no. 8; pp. 1113 - 1115
• Main Authors: SUZUKI, Jin, SASAOKA, Fumina, FUJIHARA, Masatoshi, WATANABE, Yusaku, TASAKI, Tomoko, ODA, Shinichi, KOBAYASHI, Saori, SATO, Reeko, NAGAI, Kazuya, HARASAWA, Ryô
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2011
• Subjects: Anemia, Hemolytic - epidemiology; Anemia, Hemolytic - microbiology; Anemia, Hemolytic - veterinary; Animals; Disease Outbreaks - veterinary; hemoplasma; Japan - epidemiology; Mycoplasma; Mycoplasma - genetics; Mycoplasma - isolation & purification; Mycoplasma Infections - epidemiology; Mycoplasma Infections - microbiology; Mycoplasma Infections - veterinary; ovine; Ovis aries; Phylogeny; Polymerase Chain Reaction - veterinary; Real-Time Polymerase Chain Reaction - veterinary; RNA, Bacterial - blood; RNA, Ribosomal, 16S - blood; rRNA; Sequence Analysis, RNA - veterinary; Sheep; Sheep Diseases - microbiology; Japan
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.11-0113

We examined the presence of hemoplasmas, hemotropic mycoplasmas, among 11 sheep (Ovis aries) with regenerative and hemolytic anemia and found six of them were positive by real-time PCR. The positive samples were then subjected to conventional PCR for direct sequencing of the 16S rRNA gene. Nucleotide sequences of all the positive samples were identified as the 16S rRNA gene of `Candidatus Mycoplasma haemovis' by phylogenetic analysis, demonstrating the infections with this particular hemoplasma species in Japan.