TMV resistance gene N homologues are linked to Synchytrium endobioticum resistance in potato

• Published in: Theoretical and applied genetics Vol. 98; no. 3/4; pp. 379 - 386
• Main Authors: Hehl, R, Faurie, E, Hesselbach, J, Salamini, F, Whitham, S, Baker, B, Gebbardt, C
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.03.1999; Berlin Springer Nature B.V
• Subjects: amino acid sequences; Amino acids; Antisense DNA; Arabidopsis thaliana; Binding sites; Biological and medical sciences; chromosome mapping; Classical genetics, quantitative genetics, hybrids; Cloning; comparisons; complementary DNA; Cytokines; disease resistance; Flowers & plants; Fundamental and applied biological sciences. Psychology; Fungi; genbank/aj009719; genbank/aj009720; gene location; Genes; Genetic aspects; genetic markers; genetic resistance; Genetics of eukaryotes. Biological and molecular evolution; Genomes; Identification and classification; Kinases; linkage (genetics); linkage groups; molecular sequence data; Nicotiana tabacum; nucleotide sequences; Pathogens; Plant diseases; Plant genetics; Plant immunology; Plant resistance; Polypeptides; Potatoes; Proteins; Pteridophyta, spermatophyta; restriction fragment length polymorphism; sen1 gene; Solanum tuberosum; Synchytrium endobioticum; Tobacco; Tobacco mosaic virus; Vegetals; Germany; United States > US; Genetic mapping; Linkage; Synchytrium endobioticum; Genetic marker; Plant pathogen; Nucleotide sequence; Solanum tuberosum; Phycomycetes; Tobamovirus; Homology; Fungus resistance; Fungi; Virus; Microorganism resistance; Domain structure; Tobacco mosaic virus; Dicotyledones; Angiospermae; Chromosome 11; Spermatophyta; Solanaceae; Tuber plant; Thallophyta
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051083

The fungus Synchytrium endobioticum, the causal agent of potato wart disease, is subject to worldwide quarantine regulations due to the production of persistent resting spores and lack of effective chemical control measures. The selection of Synchytrium-resistant potato cultivars may be facilitated by using markers closely linked with a resistance gene or by transferring a cloned gene for resistance into susceptible cultivars. Sen1, a gene for resistance to Synchytrium endobioticum race 1, was localized on potato chromosome XI in a genomic region which is related to the tobacco genome segment harbouring the N gene for resistance to TMV. Using N as probe, we isolated homologous cDNA clones from a Synchytrium-resistant potato line. The N-homologous sequences of potato identified by RFLP mapping a family of resistance gene-like sequences closely linked with the Sen1 locus. Sequence analysis of two full-length N-homologous cDNA clones revealed the presence of structural domains associated with resistance gene function. One clone (N1-25) encodes a polypeptide of 61 kDa and harbours a Toll-interleukin like region (TIR) and a putative nucleotide binding site (NBS). The other clone (N1-27) encodes a polypeptide of 95 kDa and harbours besides the TIR and NBS domains five imperfect leucine-rich repeats (LRRs). Both clones have at their amino terminus a conserved stretch of serine residues that was also found in the N gene, the RPPS gene from Arabidopsis thaliana and several other resistance gene homologues, suggesting a function in the resistance response. Cloning of the disease resistance locus based on map position and the establishment of PCR-based marker assays to assist selection of wart resistant potato genotypes are discussed.