Expression of the Streptomyces aureofaciens glyceraldehyde-3-phosphate dehydrogenase gene (gap) is developmentally regulated and induced by glucose

• Published in: Microbiology (Society for General Microbiology) Vol. 143; no. 11; pp. 3555 - 3561
• Main Authors: Kormanec, J, Lempel'ova, A, Novakova, R, ReZuchova, B, Homerova, D
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.1997; Society for General Microbiology
• Subjects: Amino Acid Sequence; Bacteriology; Base Sequence; Biological and medical sciences; Enzyme Induction; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial - drug effects; Gene Expression Regulation, Bacterial - genetics; Genes, Bacterial - genetics; Genes, Bacterial - physiology; Genetics; Glucose - pharmacology; Glyceraldehyde-3-Phosphate Dehydrogenases - biosynthesis; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics; Microbiology; Molecular Sequence Data; Promoter Regions, Genetic - genetics; Recombinant Fusion Proteins; Restriction Mapping; RNA, Bacterial - analysis; RNA, Messenger - analysis; Streptomyces aureofaciens; Streptomyces aureofaciens - enzymology; Streptomyces aureofaciens - genetics; Streptomycetaceae; Transcription; Enzyme; Transcription promoter; Gene organization; Gene expression; Actinomycetales; Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating); Regulation(control); Bacteria; Actinomycetes; Oxidoreductases; Streptomyces aureofaciens
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-143-11-3555

Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovak Republic 1 Author for correspondence: J. Kormanec. Tel: + 42 1 7 378 2432. Fax: +42 1 7 372 316. e-mail: umbijkor@savba.sk ABSTRACT In previous experiments, the Streptomyces aureofaciens gap gene encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was identified. To investigate expression of the gene, S1 nuclease mapping and Northern blot hybridization were performed using RNA prepared from S. aureofaciens cultivated under various conditions. These studies suggested monocistronic organization and developmental regulation of the gene. A single promoter, gap -P, was identified upstream of the gap coding region. In cultures grown on solid medium in the absence of glucose, its transcription was induced at the time of aerial mycelium formation. In addition, gap transcription was also induced in substrate mycelium by glucose. A promoter-bearing DNA fragment was inserted into two promoter-probe vectors, to give expression patterns consistent with the results of direct RNA analysis. Keywords: Streptomyces aureofaciens , differentiation, transcription, promoter, glyceraldehyde-3-phosphate dehydrogenase