Investigating the compatibility of the biocontrol agent Clonostachys rosea IK726 with prodigiosin-producing Serratia rubidaea S55 and phenazine-producing Pseudomonas chlororaphis ToZa7

• Published in: Archives of microbiology Vol. 198; no. 4; pp. 369 - 377
• Main Authors: Kamou, Nathalie N., Dubey, Mukesh, Tzelepis, Georgios, Menexes, Georgios, Papadakis, Emmanouil N., Karlsson, Magnus, Lagopodi, Anastasia L., Jensen, Dan Funck
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2016; Springer Nature B.V
• Subjects: ABC transporters; Agricultural Science; Agriculture; Antibiotics; ATP-Binding Cassette Transporters - genetics; Biochemistry; Biological control; Biomedical and Life Sciences; Biotechnology; Cell Biology; Clonostachys rosea; Drug resistance; Ecology; Fungi; Fungicides; Fusarium - physiology; Fusarium oxysporum; Gene Expression Regulation, Fungal; Genomes; Hypocreales - genetics; Hypocreales - physiology; Jordbruksvetenskap; Life Sciences; Lycopersicon esculentum; Lycopersicon esculentum - microbiology; Metabolites; Microbial Ecology; Microbial Interactions - physiology; Microbiology; Mikrobiologi; Original Paper; Pathogens; Pesticides; Plant biology; Plant Biotechnology; Plant Diseases - microbiology; Plant Diseases - prevention & control; Plant pathology; Plant Roots - microbiology; Proteins; Pseudomonas - genetics; Pseudomonas - physiology; Pseudomonas chlororaphis; Serratia - physiology; Serratia rubidaea; Tomatoes; Växtbioteknologi; Greece; Sweden; ABC transporter; Prodigiosin; Biocontrol; Pseudomonas chlororaphis; Clonostachys rosea; Serratia rubidaea
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-016-1198-4

This study was carried out to assess the compatibility of the biocontrol fungus Clonostachys rosea IK726 with the phenazine-producing Pseudomonas chlororaphis ToZa7 or with the prodigiosin-producing Serratia rubidaea S55 against Fusarium oxysporum f. sp. radicis-lycopersici . The pathogen was inhibited by both strains in vitro, whereas C . rosea displayed high tolerance to S . rubidaea but not to P . chlororaphis . We hypothesized that this could be attributed to the ATP-binding cassette (ABC) proteins. The results of the reverse transcription quantitative PCR showed an induction of seven genes ( abcB1 , abcB20 , abcB26 , abcC12 , abcC12 , abcG8 and abcG25 ) from subfamilies B, C and G. In planta experiments showed a significant reduction in foot and root rot on tomato plants inoculated with C . rosea and P . chlororaphis . This study demonstrates the potential for combining different biocontrol agents and suggests an involvement of ABC transporters in secondary metabolite tolerance in C . rosea .