Development of an antibody cocktail for treatment of Sudan virus infection
Antibody-based therapies are a promising treatment option for managing ebolavirus infections. Several Ebola virus (EBOV)-specific and, more recently, pan-ebolavirus antibody cocktails have been described. Here, we report the development and assessment of a Sudan virus (SUDV)-specific antibody cockta...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 117; no. 7; pp. 3768 - 3778 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
18.02.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Antibody-based therapies are a promising treatment option for managing ebolavirus infections. Several Ebola virus (EBOV)-specific and, more recently, pan-ebolavirus antibody cocktails have been described. Here, we report the development and assessment of a Sudan virus (SUDV)-specific antibody cocktail. We produced a panel of SUDV glycoprotein (GP)-specific human chimeric monoclonal antibodies (mAbs) using both plant and mammalian expression systems and completed head-to-head in vitro and in vivo evaluations. Neutralizing activity, competitive binding groups, and epitope specificity of SUDV mAbs were defined before assessing protective efficacy of individual mAbs using a mouse model of SUDV infection. Of the mAbs tested, GP base-binding mAbs were more potent neutralizers and more protective than glycan cap- or mucin-like domain-binding mAbs. No significant difference was observed between plant and mammalian mAbs in any of our in vitro or in vivo evaluations. Based on in vitro and rodent testing, a combination of two SUDV-specific mAbs, one base binding (16F6) and one glycan cap binding (X10H2), was down-selected for assessment in a macaque model of SUDV infection. This cocktail, RIID F6-H2, provided protection from SUDV infection in rhesus macaques when administered at 50 mg/kg on days 4 and 6 postinfection. RIID F6-H2 is an effective postexposure SUDV therapy and provides a potential treatment option for managing human SUDV infection. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: A.S.H., J.W.F., K.M., R.M.J., B.G., S.J.S., A.Z.W., S.W.S., Z.A.B., K.C., D.S., V.Y., and J.M.D. designed research; A.S.H., J.W.F., R.A.O., A.I.K., D.E.D., R.R.B., S.E.Z., N.M.J., K.M., R.M.J., S.J.S., A.Z.W., N.B., O.B., D.H.K., M.H.P., J.V., K.J.W., S.W.S., K.C., D.S., V.Y., and J.M.D. performed research; L.Z. contributed new reagents/analytic tools; A.S.H., J.W.F., R.A.O., A.I.K., D.E.D., K.M., R.M.J., B.G., S.J.S., A.Z.W., S.W.S., Z.A.B., K.C., L.Z., D.S., V.Y., and J.M.D. analyzed data; and A.S.H., J.W.F., K.M., R.M.J., B.G., S.J.S., A.Z.W., Z.A.B., K.C., L.Z., D.S., V.Y., and J.M.D. wrote the paper. 1Present address: Vaccines and Therapeutics Division, Defense Threat Reduction Agency, Fort Belvoir, VA 22060. 2Present address: Adimab, LLC, Lebanon, NH 03766. Edited by Y. Kawaoka, University of Wisconsin–Madison/University of Tokyo, Madison, WI, and approved December 30, 2019 (received for review August 28, 2019) 4Present address: Indiana Biosciences Research Institute, Indianapolis, IN 46202. 3Present address: Emergent BioSolutions, Gaithersburg, MD 20879. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.1914985117 |