Salidroside attenuates LPS-stimulated activation of THP-1 cell-derived macrophages through down-regulation of MAPK/NF-kB signaling pathways

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 33; no. 4; pp. 463 - 469
• Main Authors: Wang, Hong-wu, Wu, Ting, Qi, Jun-ying, Wang, Ya-qi, Luo, Xiao-ping, Ning, Qin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2013
• Subjects: Cell Line; Down-Regulation - drug effects; Down-Regulation - genetics; Down-Regulation - immunology; Glucosides - pharmacology; Humans; Lipopolysaccharides - immunology; Macrophages - drug effects; Macrophages - immunology; Medicine; Medicine & Public Health; Mitogen-Activated Protein Kinases - genetics; NF-kappa B - genetics; Phenols - pharmacology; Signal Transduction - drug effects; Signal Transduction - genetics; Signal Transduction - immunology; THP-1; NF-κB; salidroside; MAPK; LPS
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-013-1143-6

Summary Excessive activation of macrophages is implicated in various inflammatory injuries. Salidroside (Sal), one of the main bioactive components of Rhodiola Sachalinensis , has been reported to possess anti-inflammatory activities. This study aimed to examine the effect of Sal on the activation of macrophages and the possible mechanism. The lipopolysaccharide (LPS)-stimulated phrobol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were established. The changes in the inflammatory profiles of THP-1-derived macrophages were determined. The results showed that Sal significantly decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), interleukin-1beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) at both mRNA and protein levels in THP-1-derived macrophages, and the effect was dose-depedent. Moreover, NF-κB activation was significantly suppressed and the phosphorylation of ERK, p38 and JNK was substantially down-regulated after Sal treatment. The findings suggested that Sal can suppress the activation of LPS-stimulated PMA-differetiated THP-1 cells, as evidenced by the decreased expression of iNOS, COX2, IL-1β, IL-6 and TNF-α, and the mechanism involves the inhibition of NF-κB activation and the phosphorylation of the MAPK signal pathway.