MiR-184 Combined with STC2 Promotes Endometrial Epithelial Cell Apoptosis in Dairy Goats via RAS/RAF/MEK/ERK Pathway

• Published in: Genes Vol. 11; no. 9; p. 1052
• Main Authors: Cui, Jiuzeng, Liu, Xiaorui, Yang, Lichun, Che, Sicheng, Guo, Hongran, Han, Jincheng, Zhu, Zhongshi, Cao, Binyun, An, Xiaopeng, Zhang, Lei, Song, Yuxuan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.09.2020; MDPI
• Subjects: Animal husbandry; Animals; Apoptosis; Binding sites; Cell cycle; Cell growth; Cloning; Endometrium; Endometrium - metabolism; Endometrium - pathology; Enzymes; Epithelial cells; Epithelial Cells - metabolism; Epithelial Cells - pathology; Experiments; Extracellular signal-regulated kinase; Female; Forkhead protein; Gene expression; Gene Expression Regulation; Genetic aspects; Glycoproteins - genetics; Glycoproteins - metabolism; Goats; Health aspects; Liver cancer; Livestock; Mammals; MAP Kinase Signaling System; Metabolic pathways; MicroRNA; MicroRNAs - genetics; miRNA; Physiological aspects; Pregnancy; Proteins; raf Kinases - genetics; raf Kinases - metabolism; ras Proteins - genetics; ras Proteins - metabolism; Vascular endothelial growth factor; China; United States > US; endometrial epithelial cells (EECs); apoptosis; miR-184; receptive endometrium; STC2; dairy goats
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes11091052

The endometrium undergoes a series of complex changes to form a receptive endometrium (RE) that allows the embryo to be implanted. The inability to establish endometrial receptivity of livestock causes embryo implantation failure and considerable losses to animal husbandry. MicroRNAs (miRNAs) are a class of noncoding RNAs. Studies have found that miRNAs can regulate many critical physiological processes, including the establishment of RE during embryo implantation. miR-184 is highly expressed in the endometrial receptive period of dairy goats. This study aimed to explore the effect of miR-184 on endometrial epithelial cell (EEC) apoptosis and RE establishment. Stanniocalcin2 (STC2) is a direct target of miR-184, and miR-184 decreases the expression of STC2 in dairy goat EECs. miR-184 can activate EECs apoptosis through the RAS/RAF/MEK/ERK pathway. Additionally, miR-184 increases the expression levels of RE marker genes, such as forkhead box M1 (FOXM1) and vascular endothelial growth factor (VEGF). These findings indicate that miR-184 promotes the apoptosis of endometrial epithelial cells in dairy goats by downregulating STC2 via the RAS/RAF/MEK/ERK pathway, and that it may also regulate the establishment of RE in dairy goats.