Molecularly imprinted polymer using β-cyclodextrin as functional monomer for the efficient recognition of bilirubin

• Published in: Analytica chimica acta Vol. 606; no. 1; pp. 92 - 97
• Main Authors: Yang, Yu, Long, Yuanyuan, Cao, Qing, Li, Kean, Liu, Feng
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07.01.2008; Elsevier
• Subjects: Adsorption; Analytical chemistry; beta-Cyclodextrins - chemistry; Bilirubin; Bilirubin - blood; Bilirubin - chemistry; Chemistry; Exact sciences and technology; Humans; Kinetics; Molecular Imprinting - methods; Molecular Structure; Molecularly imprinted polymer; Polymers - chemistry; Reproducibility of Results; Selectivity; Sensitivity and Specificity; Spectrometric and optical methods; Testosterone - chemistry; β-Cyclodextrin; β-Cyclodextrin; Selectivity; Bilirubin; Molecularly imprinted polymer; Human; Molecular structure; Hydrogen; Polymer; Chemical enrichment; Absorption spectrometry; Testosterone; Cyclodextrin; Adsorption; Serum; Molecular imprinting; Ultraviolet visible spectrometry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2007.10.044

Bilirubin (BR) imprinted polymer was successfully prepared using supramolecular host compound β-cyclodextrin (β-CD) as functional monomer. The adsorption equilibrium was attained in about 4 h, which indicated that the adsorption kinetics was comparatively fast. The results of adsorption and selectivity experiments indicated that BR-imprinted β-CD polymer was able to bind BR specifically and reversibly. The specific recognition of BR-imprinted β-CD polymer for BR may be due to the cooperative effects of inclusion interaction and hydrogen bonding. This BR-imprinted β-CD polymer was further applied to eliminate BR in human serum sample. It was verified that the binding specificity of the BR-imprinted polymer for BR was essentially sufficient in the presence of other compounds coexisting in serum sample. Therefore, as a reusable material possessing high affinity and selectivity, BR-imprinted β-CD polymer has a potential application perspective as a clinical hemoperfusion material.