Expression of potyvirus coat protein in Escherichia coli and yeast and its assembly into virus-like particles
CSIRO, Division of Biomolecular Engineering, Parkville Laboratory, 343 Royal Parade, Parkville 3052, Victoria, Australia When the full-length coat protein (CP) of the potyvirus, Johnsongrass mosaic virus (JGMV), was expressed in Escherichia coli or yeast, it assembled to form potyvirus-like particle...
Saved in:
Published in | Journal of general virology Vol. 72; no. 7; pp. 1543 - 1550 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Reading
Soc General Microbiol
01.07.1991
Society for General Microbiology |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | CSIRO, Division of Biomolecular Engineering, Parkville Laboratory, 343 Royal Parade, Parkville 3052, Victoria, Australia
When the full-length coat protein (CP) of the potyvirus, Johnsongrass mosaic virus (JGMV), was expressed in Escherichia coli or yeast, it assembled to form potyvirus-like particles. The particles were heterogeneous in length with a stacked-ring appearance and resembled JGMV particles in their flexuous morphology and width. This cell-free assembly system should permit analysis of the mechanisms of particle assembly and genome encapsidation. Two mutant forms of CP produced by site-directed mutagenesis failed to assemble into virus-like particles.
Received 14 December 1990;
accepted 29 March 1991. |
---|---|
ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-72-7-1543 |