Differential Regulation of Circadian Pacemaker Output by Separate Clock Genes in Drosophila

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 97; no. 7; pp. 3608 - 3613
• Main Authors: Park, Jae H., Helfrich-Forster, Charlotte, Lee, Gyunghee, Liu, Li, Rosbash, Michael, Hall, Jeffrey C.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 28.03.2000; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences
• Subjects: Animals; Animals, Genetically Modified; Base Sequence; Biological Clocks - genetics; Biological Sciences; Brain; Brain - metabolism; Cerebral hemispheres; Chronobiology; Circadian rhythm; CLOCK protein; CYCLE protein; DNA; Drosophila; Drosophila - genetics; Drosophila - physiology; Drosophila melanogaster; Drosophila Proteins; Gene Expression Regulation; Genes; Genetic mutation; Immunohistochemistry; In Situ Hybridization; Insect Proteins - genetics; Insect Proteins - metabolism; Insects; Larvae; Messenger RNA; Mutation; Neurology; Neurons; neuropeptides; Neuropeptides - genetics; Neuropeptides - metabolism; period gene; pigment-dispersing factor (pdf) gene; RNA Processing, Post-Transcriptional; RNA, Messenger - genetics; timeless gene
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.070036197

Regulation of the Drosophila pigment-dispersing factor (pdf) gene products was analyzed in wild-type and clock mutants. Mutations in the transcription factors CLOCK and CYCLE severely diminish pdf RNA and neuropeptide (PDF) levels in a single cluster of clock-gene-expressing brain cells, called small ventrolateral neurons (s-LNvS). This clock-gene regulation of specific cells does not operate through an E-box found within pdf regulatory sequences. PDF immunoreactivity exhibits daily cycling, but only within terminals of axons projecting from the s-LNvS. This posttranslational rhythm is eliminated by period or timeless null mutations, which do not affect PDF staining in cell bodies or pdf mRNA levels. Therefore, within these chronobiologically important neurons, separate elements of the central pacemaking machinery regulate pdf or its product in novel and different ways. Coupled with contemporary results showing a pdf-null mutant to be severely defective in its behavioral rhythmicity, the present results reveal PDF as an important circadian mediator whose expression and function are downstream of the clockworks.