Photosynthetic electron transport adjustments in overwintering Scots pine (Pinus sylvestris L.)

• Published in: Planta Vol. 213; no. 4; pp. 575 - 585
• Main Authors: Ivanov, A.G., Sane, P.V., Zeinalov, Y., Malmberg, G., Gardeström, P., Huner, N.P.A., Öquist, G.
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01.08.2001; Springer
• Subjects: Adaptation, Physiological; Adenosine Diphosphate - metabolism; Adenosine Triphosphate - metabolism; Agronomy. Soil science and plant productions; Biological and medical sciences; Chlorophyll - metabolism; Chlorophylls; Chloroplasts; Economic plant physiology; Electron Transport; Fluorescence; Fundamental and applied biological sciences. Psychology; Kinetics; Light-Harvesting Protein Complexes; Metabolism; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Oxidation-Reduction; P700; Photochemistry; Photosynthesis; Photosynthesis - physiology; Photosynthesis, respiration. Anabolism, catabolism; Photosynthetic Reaction Center Complex Proteins - metabolism; Pine trees; Pinus (winter stress); Pinus - physiology; Pinus sylvestris; Plant Leaves - physiology; Plant physiology and development; Plants; Plastoquinone - metabolism; Seasons; Summer; Temperature; thermoluminescence; Winter; winter stress; Overwintering; Electron transfer; Plant leaf; Pinus sylvestris; Stress; Photosystem; Cyclic electron transfer; Softwood forest tree; Gymnospermae; Coniferales; Spermatophyta; Photosynthesis; Photoprotection; Photochemistry
• ISSN: 0032-0935; 1432-2048; 1432-2048
• DOI: 10.1007/s004250100522

As shown before [C. Ottander et al. (1995) Planta 197:176—183], there is a severe inhibition of the photosystem (PS) II photochemical efficiency of Scots pine (Pinus sylvestris L.) during the winter. In contrast, the in vivo PSI photochemistry is less inhibited during winter as shown by in vivo measurements of ΔA820/A820 (P700+). There was also an enhanced cyclic electron transfer around PSI in winter-stressed needles as indicated by 4-fold faster reduction kinetics of P700+. The differential functional stability of PSII and PSI was accompanied by a 3.7-fold higher intersystem electron pool size, and a 5-fold increase in the stromal electron pool available for P700+ reduction. There was also a strong reduction of the QB band in the thermoluminescence glow curve and markedly slower ${\mathrm{Q}}_{\mathrm{A}}^{-}$ re-oxidation in needles of winter pine, indicating an inhibition of electron transfer between QA and QB. The data presented indicate that the plastoquinone pool is largely reduced in winter pine, and that this reduced state is likely to be of metabolic rather than photochemical origin. The retention of PSI photochemistry, and the suggested metabolic reduction of the plastoquinone pool in winter stressed needles of Scots pine are discussed in terms of the need for enhanced photoprotection of the needles during the winter and the role of metabolically supplied energy for the recovery of photosynthesis from winter stress in evergreens.