Surface enhanced Raman spectroscopic monitor of P. acnes lipid hydrolysis in vitro

Surface enhanced Raman spectroscopy (SERS) at a silver microelectrode was used to monitor bacterial hydrolysis of triglycerides in lipid mixtures that model sebaceous gland secretions. Mixtures of wax esters, squalene, triolein, and triisostearin were used as model skin secretions. The transformatio...

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Bibliographic Details
Published inJournal of lipid research Vol. 39; no. 9; pp. 1896 - 1899
Main Authors Weldon, M K, Morris, M D, Harris, A B, Stoll, J K
Format Journal Article
LanguageEnglish
Published United States Elsevier 01.09.1998
Subjects
Esters - metabolism
fatty acids
Hydrolysis
Lipid Metabolism
lipids
microelectrode
Models, Biological
Propionibacterium acnes - metabolism
Raman spectroscopy
Sebaceous Glands - metabolism
Spectrum Analysis, Raman - methods
Squalene - metabolism
surface-enhanced
triglycerides
Triglycerides - metabolism
Triolein - metabolism
Waxes - metabolism
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Summary:Surface enhanced Raman spectroscopy (SERS) at a silver microelectrode was used to monitor bacterial hydrolysis of triglycerides in lipid mixtures that model sebaceous gland secretions. Mixtures of wax esters, squalene, triolein, and triisostearin were used as model skin secretions. The transformation was followed in vitro as changes in the SERS caused by hydrolysis of triglyceride to fatty acid. The fatty acid was adsorbed as its carboxylate, which is readily identified by the characteristic band at ca. 1395 cm(-1). Co-adsorption of propionate was also observed. The technique can also confirm the presence of bacteria by detection of short chain carboxylic acids released as products of fermentation during the growth of these cells.
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ISSN:0022-2275
DOI:10.1016/S0022-2275(20)32178-7