Evolution of insulin at the edge of foldability and its medical implications

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 117; no. 47; pp. 29618 - 29628
• Main Authors: Rege, Nischay K., Liu, Ming, Yang, Yanwu, Dhayalan, Balamurugan, Wickramasinghe, Nalinda P., Chen, Yen-Shan, Rahimi, Leili, Guo, Huan, Haataja, Leena, Sun, Jinhong, Ismail-Beigi, Faramarz, Phillips, Nelson B., Arvan, Peter, Weiss, Michael A.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 24.11.2020
• Subjects: Amino Acid Substitution - physiology; Animals; Beta cells; Biological Sciences; Biosynthesis; Cell death; Cell Line; Cell Line, Tumor; Cystine; Diabetes mellitus; Diabetes Mellitus - metabolism; Disulfide bonds; Disulfides - metabolism; Endoplasmic reticulum; Evolution; Folding; Gene Regulatory Networks - physiology; Genetic diversity; Growth factors; HEK293 Cells; Humans; Hydroxyl groups; Insulin; Insulin - metabolism; Insulin-like growth factors; Insulin-Secreting Cells - metabolism; MCF-7 Cells; Mutation; Pandemics; Proinsulin - metabolism; Protein Binding - physiology; Protein Folding; Protein transport; Proteins; Rats; Receptor, Insulin - metabolism; Receptors; Secretion; Self-assembly; Structure-Activity Relationship; Vertebrates; protein structure; unfolded protein response; protein folding; folding efficiency; evolutionary medicine
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2010908117

Proteins have evolved to be foldable, and yet determinants of foldability may be inapparent once the native state is reached. Insight has emerged from studies of diseases of protein misfolding, exemplified by monogenic diabetes mellitus due to mutations in proinsulin leading to endoplasmic reticulum stress and β-cell death. Cellular foldability of human proinsulin requires an invariant Phe within a conserved crevice at the receptor-binding surface (position B24). Any substitution, even related aromatic residue TyrB24, impairs insulin biosynthesis and secretion. As a seeming paradox, a monomeric TyrB24 insulin analog exhibits a native-like structure in solution with only a modest decrement in stability. Packing of TyrB24 is similar to that of PheB24, adjoining core cystine B19–A20 to seal the core; the analog also exhibits native self-assembly. Although affinity for the insulin receptor is decreased ∼20-fold, biological activities in cells and rats were within the range of natural variation. Together, our findings suggest that the invariance of PheB24 among vertebrate insulins and insulin-like growth factors reflects an essential role in enabling efficient protein folding, trafficking, and secretion, a function that is inapparent in native structures. In particular, we envision that the para-hydroxyl group of TyrB24 hinders pairing of cystine B19–A20 in an obligatory on-pathway folding intermediate. The absence of genetic variation at B24 and other conserved sites near this disulfide bridge—excluded due to β-cell dysfunction—suggests that insulin has evolved to the edge of foldability. Nonrobustness of a protein’s fitness landscape underlies both a rare monogenic syndrome and “diabesity” as a pandemic disease of civilization.