Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray

• Published in: Theoretical and applied genetics Vol. 94; no. 2; pp. 151 - 158
• Main Authors: Dillen, W, Clercq, J. de, Goossens, A, Montagu, M. van, Angenon, G. (Gent Univ. (Belgium). Flanders Interuniv. Inst. for Biotechnology. Dept. of Genetics. Lab. voor Genetica)
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.02.1997; Berlin Springer Nature B.V
• Subjects: AGROBACTERIUM TUMEFACIENS; Beans; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Common beans; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetic aspects; GENETIC TRANSFORMATION; Genetically modified plants; Genetics; Genetics of eukaryotes. Biological and molecular evolution; PEST RESISTANCE; PHASEOLUS ACUTIFOLIUS; Phaseolus vulgaris; Plant genetics; Pteridophyta, spermatophyta; RESISTANCE AUX ORGANISMES NUISIBLES; RESISTENCIA A LAS PLAGAS; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Vegetals; ZABROTES SUBFASCIATUS; Belgium; Storage protein; Genetic transformation; Coleoptera; Insecta; Agrobacterium tumefaciens; Tissue culture; Gene expression; Phaseolus acutifolius; Grain legume; Regeneration; Zabrotes subfasciatus; Pest; Reporter gene; Leguminosae; Arthropoda; Dicotyledones; Insect resistance; Angiospermae; Bruchidae; Bacteria; Spermatophyta; Transgenic plant; Rhizobiaceae; Invertebrata
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050394

Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray has been achieved. Regeneration-competent callus, obtained from bud explants of greenhouse-grown plants, was co-cultivated with Agrobacterium tumefaciens C58C1[Rif.sup.R] (pMP90) harbouring a binary vector with the neomycin phosphotransferase II (nptII) and β-glucuronidase (uidA) marker genes. Transient expression of uidA was detected in five out of six genotypes tested. Transgenic callus lines of three genotypes were established on geneticin-containing medium. Plants were recovered from one line (genotype NI 576). This line had been transformed with a binary plasmid which, in addition to the marker genes, contained a genomic fragment encoding the Phaseolus vulgaris arcelin-5a protein. This seed storage protein presumably confers resistance to the insect Zabrotes subfasciatus, a major pest of P. vulgaris. Integration of foreign DNA was confirmed by molecular analysis. The introduced genes segregated as a single locus. Arcelin-5a was produced at high levels in seeds. The possibility of using P. acutifolius as a 'bridging' species to introduce transgenes into the economically more important species P. vulgaris is discussed.