Production of validamycin A from hemicellulose hydrolysate by Streptomyces hygroscopicus 5008

• Published in: Bioresource technology Vol. 175; pp. 160 - 166
• Main Authors: Zhou, Tan-Che, Zhong, Jian-Jiang
• Format: Journal Article
• Language: English
• Published: England 01.01.2015
• Subjects: Antibiotics; Arabinose - metabolism; Carbon; Carbon - metabolism; Cost engineering; Fermentation; Genetic engineering; Glucose - metabolism; Hydrolysates; Hydrolysis; Industrial Microbiology - methods; Inositol - analogs & derivatives; Inositol - biosynthesis; Polysaccharides - metabolism; Strain; Streptomyces - genetics; Streptomyces - metabolism; Streptomyces hygroscopicus; Xylose - metabolism; Zea mays - chemistry; Antibiotics fermentation; Streptomyces hygroscopicus; Validamycin A; d-Xylose; Hemicellulose hydrolysate
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.10.051

Validamycin A (VAL-A) is an important agricultural antibiotic produced by Streptomyces hygroscopicus 5008, which uses starch as carbon source occupying about 20% of total production cost. To reduce the medium cost, corncob hydrolysate - a hemicellulose hydrolysate was applied as a low-cost substrate to VAL-A fermentation. It was found that three major sugars in corncob hydrolysate including d-glucose, d-xylose and l-arabinose could all be utilized by S. hygroscopicus 5008 to produce VAL-A while d-xylose was the main contributor. A higher VAL-A production titer from d-xylose was achieved by using a genetically engineered strain TC03 derived from S. hygroscopicus 5008, which resulted in 1.27-fold improvement of VAL-A production from the medium containing 13% (v/v) corncob hydrolysate compared to that by its original strain. A medium cost analysis was done and compared with previous reports. This work indicates a great potential of the hemicellulose hydrolysate as substrate for antibiotic fermentation.