Glucosamine content of tempe mould, Rhizopus oligosporus

• Published in: International journal of food microbiology Vol. 47; no. 1; pp. 153 - 157
• Main Authors: Sparringa, R.A, Owens, J.D
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.03.1999; Elsevier
• Subjects: ALIMENT A BASE DE SOJA POUR HOMME; ALIMENTOS DERIVADOS DE LA SOJA; AMINO SUGARS; AMINOAZUCARES; AMINOSUCRE; Biological and medical sciences; BIOLOGICAL PRODUCTION; Biomass; Biomass estimation; CHITIN; CHITINE; Culture Media; FERMENTACION EN ESTADO SOLIDO; FERMENTATION A L'ETAT SOLIDE; Fermented food industries; Food industries; Fundamental and applied biological sciences. Psychology; Glucosamine; Glucosamine - analysis; Glycine max - microbiology; Other fermented food industries; PRODUCCION BIOLOGICA; PRODUCTION BIOLOGIQUE; QUITINA; Rhizopus - chemistry; Rhizopus - growth & development; Rhizopus - metabolism; RHIZOPUS OLIGOSPORUS; SOLID STATE FERMENTATION; Solid substrate fermentation; SOYFOODS; Spores, Fungal - growth & development; Tempe; Tempeh; Asia; Indonesia; Glucosamine; Biomass estimation; Solid substrate fermentation; Chitin; Rhizopus oligosporus; Tempe; Tempeh; Fungi; Phycomycetes; Biomass; Traditional food stuff; Thallophyta; Soybean cake
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/S0168-1605(99)00020-3

The glucosamine content of Rhizopus oligosporus NRRL 2710 mycelium grown in different media was determined. In Sabouraud dextrose broth the glucosamine content ranged from 51 g (kg dry biomass) −1 for mycelial pellets less than 5 mm diameter to 107 g kg −1 for pellets 16–35 mm diameter. Mycelium grown on Sabouraud dextrose agar contained 111 g glucosamine (kg dry biomass) −1 while that grown on soymilk agar, used to simulate growth on soybeans in tempe, contained 82 g kg −1. The estimation of glucosamine was reproducible, with a mean coefficient of variation of 4% for mycelial pellets and 11% for mycelium from agar media. It is suggested that a conversion factor of 12 g dry biomass (g glucosamine) −1 is applicable to determine fungal biomass in tempe fermentation.