Nucleotide excision repair removes thymidine analog 5-ethynyl-2′-deoxyuridine from the mammalian genome

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 119; no. 35; pp. 1 - 8
• Main Authors: Wang, Li, Cao, Xuemei, Yang, Yanyan, Kose, Cansu, Kawara, Hiroaki, Lindsey-Boltz, Laura A., Selby, Christopher P., Sancar, Aziz
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 30.08.2022
• Subjects: Adducts; Antineoplastic drugs; Antitumor agents; Biological Sciences; Blood-brain barrier; Carcinogens; Cisplatin; Deoxyribonucleic acid; Deoxyuridine - analogs & derivatives; DNA; DNA - chemistry; DNA - genetics; DNA adducts; DNA Damage; DNA Repair; Genome, Human; Genomes; Humans; Mammals; Nucleotide excision repair; Nucleotides; Repair; Substrates; Thymidine; Thymidine - analogs & derivatives; Toxicity; Transcription, Genetic; Transcription-coupled repair; Ultraviolet radiation; Ultraviolet Rays; XR-seq; 5-ethynyl-2′-deoxyuridine; excision repair; brain cancer
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2210176119

Nucleotide excision repair is the principal mechanism for removing bulky DNA adducts from the mammalian genome, including those induced by environmental carcinogens such as UV radiation, and anticancer drugs such as cisplatin. Surprisingly, we found that the widely used thymidine analog EdU is a sub...