Ultrasound-Assisted Enzyme Extraction, Physicochemical Properties and Antioxidant Activity of Polysaccharides from Cordyceps militaris Solid Medium

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 19; p. 4560
• Main Authors: Wang, Xiaoya, Zhang, Jingyan, Zhang, Kang, Guo, Zhiting, Xu, Guowei, Huang, Liping, Wang, Lei, Li, Jianxi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.09.2024; MDPI
• Subjects: Analysis; antioxidant; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Biological activity; Cellulase; Cellulase - chemistry; Cellulase - metabolism; Cordyceps - chemistry; Cordyceps militaris solid medium; Dextrose; Enzymes; Fructose; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - isolation & purification; Fungal Polysaccharides - pharmacology; Glucose; Infrared spectroscopy; Molecular Weight; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; properties; Solvents; Spectroscopy, Fourier Transform Infrared; Spectrum analysis; Temperature; Ultrasonic imaging; Ultrasonic Waves; ultrasound-assisted enzyme; Congo (Kinshasa); China; polysaccharides; ultrasound-assisted enzyme; antioxidant; Cordyceps militaris solid medium; properties
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29194560

solid medium polysaccharides (CMMPs) were extracted using an ultrasound-assisted enzyme method, and the process conditions were optimized via response surface methodology (RSM). The CMMPs were separated into four components named CMMP-1, CMMP-2, CMMP-3 and CMMP-4 using ethanol fractional precipitation, and their monosaccharide composition and structural properties were analyzed by molecular weight analysis, Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), Congo red test, ultraviolet-visible spectroscopy (UV-vis), atomic force microscopy (AFM), and thermogravimetric analysis (TGA). RSM could predict the yield of the CMMP (R = 0.9928), and the polysaccharide yield was 15.43% under the selected conditions of 3.1% cellulase enzyme addition, a liquid-solid ratio of 42:1, an extraction temperature of 61 °C, and an extraction time of 60 min. Glucose and galactose were the main constituents of the four fractional precipitated polysaccharides. Furthermore, four components exhibited antioxidant activity, and CMMP-1 demonstrated stronger antioxidant activity in vitro. This study demonstrates the possibility of developing a natural antioxidant food from solid medium.