Design of a Multiepitope Vaccine against Chicken Anemia Virus Disease

Chicken anemia virus (CAV) causes severe clinical and sub-clinical infection in poultry globally and thus leads to economic losses. The drawbacks of the commercially available vaccines against CAV disease signal the need for a novel, safe, and effective vaccine design. In this study, a multiepitope...

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Published inViruses Vol. 14; no. 7; p. 1456
Main Authors Fatoba, Abiodun Joseph, Adeleke, Victoria T, Maharaj, Leah, Okpeku, Moses, Adeniyi, Adebayo A, Adeleke, Matthew A
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 30.06.2022
MDPI
Subjects
Anemia
Antibodies
Antigenic determinants
Antigens
Bioinformatics
chicken anemia virus
Cloning vectors
Design and construction
disease
Diseases
DNA virus infections
Epitopes
Immune response
immunoinformatics
Infections
Lymphocytes
Lymphocytes B
Lymphocytes T
Molecular dynamics
multiepitope
Poultry
Prevention
Proteins
Testing
TLR3 protein
Toll-like receptors
Vaccines
viral proteins
Viral vaccines
Viruses
VP1 protein
South Africa
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Summary:Chicken anemia virus (CAV) causes severe clinical and sub-clinical infection in poultry globally and thus leads to economic losses. The drawbacks of the commercially available vaccines against CAV disease signal the need for a novel, safe, and effective vaccine design. In this study, a multiepitope vaccine (MEV) consisting of T-cell and B-cell epitopes from CAV viral proteins (VP1 and VP2) was computationally constructed with the help of linkers and adjuvant. The 3D model of the MEV construct was refined and validated by different online bioinformatics tools. Molecular docking showed stable interaction of the MEV construct with TLR3, and this was confirmed by Molecular Dynamics Simulation. Codon optimization and in silico cloning of the vaccine in pET-28a (+) vector also showed its potential expression in the E. coli K12 system. The immune simulation also indicated the ability of this vaccine to induce an effective immune response against this virus. Although the vaccine in this study was computationally constructed and still requires further in vivo study to confirm its effectiveness, this study marks a very important step towards designing a potential vaccine against CAV disease.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v14071456