Utilization of Grape Seed Flour for Antimicrobial Lipopeptide Production by Bacillus amyloliquefaciens C5 Strain

• Published in: Applied biochemistry and biotechnology Vol. 187; no. 4; pp. 1460 - 1474
• Main Authors: Soussi, Siwar, Essid, Rym, Hardouin, Julie, Gharbi, Dorra, Elkahoui, Salem, Tabbene, Olfa, Cosette, Pascal, Jouenne, Thierry, Limam, Ferid
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2019; Springer Nature B.V; Humana Press
• Subjects: Anti-Infective Agents - metabolism; Anti-Infective Agents - pharmacology; Antibacterial activity; Antimicrobial activity; Antimicrobial agents; Bacillomycin; Bacillus amyloliquefaciens; Bacillus amyloliquefaciens - metabolism; Biochemistry; Biochemistry, Molecular Biology; Biosurfactants; Biotechnology; Biotechnology - methods; Chemistry; Chemistry and Materials Science; Endophytes; Flour; Fruit trees; Grapes; Life Sciences; Lipopeptides; Lipopeptides - biosynthesis; Lipopeptides - pharmacology; Microbial Sensitivity Tests; Minimum inhibitory concentration; Optimization; Seeds - chemistry; Substrates; Surfactin; Thin layer chromatography; Vitis - chemistry; MALDI-TOF/MS; Antimicrobial activity; Grape seed flour; M9 mineral medium; Lipopeptide; Bacillus amyloliquefaciens
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-018-2885-1

An endophytic Bacillus amyloliquefaciens strain called C5, able to produce biosurfactant lipopeptides with a broad antibacterial activity spectrum, has been isolated from the roots of olive tree. Optimization of antibacterial activity was undertaken using grape seed flour (GSF) substrate at 0.02, 0.2, and 2% ( w / v ) in M9 medium. Strain C5 exhibited optimal growth and antimicrobial activity (MIC value of 60 μg/ml) when incubated in the presence of 0.2% GSF while lipopeptide production culminated at 2% GSF. Thin layer chromatography analysis of lipopeptide extract revealed the presence of at least three active spots at Rf 0.35, 0.59, and 0.72 at 0.2% GSF. Data were similar to those obtained in LB-rich medium. MALDI-TOF/MS analysis of lipopeptide extract obtained from 0.2% GSF substrate revealed the presence of surfactin and bacillomycin D. These results show that GSF could be used as a low-cost culture medium supplement for optimizing the production of biosurfactants by strain C5.