Immunohistochemical detection of avian pneumovirus in formalin-fixed tissues

• Published in: Journal of veterinary diagnostic investigation Vol. 13; no. 1; p. 13
• Main Authors: Jirjis, F E, Noll, S L, Halvorson, D A, Nagaraja, K V, Shaw, D P
• Format: Journal Article
• Language: English
• Published: United States 01.01.2001
• Subjects: Animals; Antigens, Viral - analysis; Female; Formaldehyde; Immunohistochemistry; Male; Nasal Mucosa - virology; Pneumovirus - immunology; Pneumovirus - isolation & purification; Pneumovirus Infections - diagnosis; Pneumovirus Infections - veterinary; Poultry Diseases - diagnosis; Rabbits - immunology; Tissue Fixation; Turkeys

An immunohistochemical staining technique (IHC) was developed to detect avian pneumovirus (APV) antigen in formalin-fixed, paraffin-embedded tissue sections using streptavidin-biotin immunoperoxidase staining. Samples of nasal turbinates and infraorbital sinuses were collected from 4-week-old poults...