Immunohistochemical detection of avian pneumovirus in formalin-fixed tissues

An immunohistochemical staining technique (IHC) was developed to detect avian pneumovirus (APV) antigen in formalin-fixed, paraffin-embedded tissue sections using streptavidin-biotin immunoperoxidase staining. Samples of nasal turbinates and infraorbital sinuses were collected from 4-week-old poults...

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Bibliographic Details
Published inJournal of veterinary diagnostic investigation Vol. 13; no. 1; p. 13
Main Authors Jirjis, F E, Noll, S L, Halvorson, D A, Nagaraja, K V, Shaw, D P
Format Journal Article
LanguageEnglish
Published United States 01.01.2001
Subjects
Animals
Antigens, Viral - analysis
Female
Formaldehyde
Immunohistochemistry
Male
Nasal Mucosa - virology
Pneumovirus - immunology
Pneumovirus - isolation & purification
Pneumovirus Infections - diagnosis
Pneumovirus Infections - veterinary
Poultry Diseases - diagnosis
Rabbits - immunology
Tissue Fixation
Turkeys
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Summary:An immunohistochemical staining technique (IHC) was developed to detect avian pneumovirus (APV) antigen in formalin-fixed, paraffin-embedded tissue sections using streptavidin-biotin immunoperoxidase staining. Samples of nasal turbinates and infraorbital sinuses were collected from 4-week-old poults experimentally inoculated with APV and from older turkeys infected during naturally occurring outbreaks of avian pneumovirus. Tissue was fixed in 10% buffered neutral formalin, embedded in paraffin, sectioned and stained. Inflammatory changes were observed microscopically in the mucosa and submucosa of the nasal turbinates and infraorbital sinuses of both experimentally inoculated poults and naturally infected birds. Viral antigen was detected by IHC in the ciliated epithelial cells of nasal turbinates and infraorbital sinuses.
ISSN:1040-6387
DOI:10.1177/104063870101300103