Analysis and Reproducibility of 3′-Deoxy-3′-[18F]Fluorothymidine Positron Emission Tomography Imaging in Patients with Non–Small Cell Lung Cancer

• Published in: Clinical cancer research Vol. 14; no. 14; pp. 4463 - 4468
• Main Authors: Shields, Anthony F, Lawhorn-Crews, Jawana M, Briston, David A, Zalzala, Sajad, Gadgeel, Shirish, Douglas, Kirk A, Mangner, Thomas J, Heilbrun, Lance K, Muzik, Otto
• Format: Journal Article
• Language: English
• Published: United States American Association for Cancer Research 15.07.2008
• Subjects: Aged; cancer; Carcinoma, Non-Small-Cell Lung - diagnostic imaging; Dideoxynucleosides - pharmacokinetics; Female; FLT; Humans; Image Processing, Computer-Assisted; Lung Neoplasms - diagnostic imaging; Male; Middle Aged; positron emission tomography; Positron-Emission Tomography - methods; Radiopharmaceuticals - pharmacokinetics; reproducibility; Reproducibility of Results
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.CCR-07-5243

Purpose: Imaging tumor proliferation with 3′-deoxy-3′-[ 18 F]fluorothymidine (FLT) and positron emission tomography is being developed with the goal of monitoring antineoplastic therapy. This study assessed the methods to measure FLT retention in patients with non-small cell lung cancer (NSCLC) to measure the reproducibility of this approach. Experimental Design: Nine patients with NSCLC who were untreated or had progressed after previous therapy were imaged twice using FLT and positron emission tomography within 2 to 7 days. Reproducibility (that is, error) was measured as the percent difference between the two patient scans. Dynamic imaging was obtained during the first 60 min after injection. Activity in the blood was assessed from aortic images and the fraction of unmetabolized FLT was measured. Regions of interest were drawn on the plane with the highest activity and the adjacent planes to measure standardized uptake value (SUV mean ) and kinetic variables of FLT flux. Results: We found that the SUV mean obtained from 30 to 60 min had a mean error of 3.6% (range, 0.6-6.9%; SD, 2.3%) and the first and second scans were highly correlated ( r 2 = 0.99; P < 0.0001). Using shorter imaging times from 25 to 30 min or from 55 to 60 min postinjection also resulted in small error rates; SUV mean mean errors were 8.4% and 5.7%, respectively. Compartmental and graphical kinetic analyses were also fairly reproducible ( r 2 = 0.59; P = 0.0152 and r 2 = 0.58; P = 0.0175 respectively). Conclusion: FLT imaging of patients with NSCLC was quite reproducible with a worst case SUV mean error of 21% when using a short imaging time.