Isolation of Extracellular Vesicles from Stem Cells

Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncodi...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1660; p. 389
Main Authors Chen, Zixin, Li, Yongjun, Yu, Hong, Shen, Yan, Ju, Chengwei, Ma, Genshan, Liu, Yutao, Kim, Il-Man, Weintraub, Neal L, Tang, Yaoliang
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Animals
Cells, Cultured
Extracellular Vesicles - chemistry
Extracellular Vesicles - metabolism
Extracellular Vesicles - ultrastructure
Fluorescent Antibody Technique
Induced Pluripotent Stem Cells - metabolism
Mice
Particle Size
Stem Cells - metabolism
Ultracentrifugation - methods
Extracellular vesicles
Induced pluripotent stem cells
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Summary:Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia-reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .
ISSN:1940-6029
DOI:10.1007/978-1-4939-7253-1_32