Reactivity of different LH and FSH standards and preparations in the World Health Organization matched reagents for enzyme-linked immunoassays of gonadotrophins

• Published in: Human reproduction (Oxford) Vol. 15; no. 11; pp. 2285 - 2291
• Main Authors: Olivares, A., Cárdenas, M., Timossi, C., Zariñán, T., Díaz-Sánchez, V., Ulloa-Aguirre, A.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.11.2000
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Calibration; CHO Cells; Cricetinae; Dose-Response Relationship, Drug; enzyme-linked immunoassay; Enzyme-Linked Immunosorbent Assay - methods; Follicle Stimulating Hormone - blood; Follicle Stimulating Hormone - standards; FSH; Fundamental and applied biological sciences. Psychology; Humans; Luteinizing Hormone - blood; Luteinizing Hormone - standards; Pituitary Gland - metabolism; Protein hormones. Growth factors. Cytokines; Proteins; Reagent Kits, Diagnostic; Recombinant Proteins; Reference Standards; World Health Organization; LH; enzyme-linked immunoassay; FSH; Human; Methodology; Rodentia; Gonadotropin; Enzyme immunoassay; Dose activity relation; Ovary; Vertebrata; Cell line; Mammalia; Adenohypophyseal hormone; Animal; Female genital system; Luteinizing hormone; Recombinant protein; Hamster; Follicle stimulating hormone
• ISSN: 0268-1161; 1460-2350; 1460-2350
• DOI: 10.1093/humrep/15.11.2285

The immunoreactivity of various LH and FSH calibration standards and recombinant preparations in the enzyme-linked immunoassay (EIA) systems for gonadotrophins developed for the Special Programme of Research in Human Reproduction of the World Health Organization (WHO) were compared. The preparations tested included three LH and two FSH pituitary standards (calibrated against LH 80/552 and 68/40 and FSH 78/549 respectively) provided with the EIA or radioimmunoassay WHO matched reagent kits, the pituitary preparation LER-907, and recombinant human LH (rhLH) and FSH (rhFSH). Simultaneous curve fitting of the EIA dose-response curves revealed no significant differences among the slopes generated by the WHO LH standards and LER-907; in contrast, no parallelism was found between the curves of rhLH and the pituitary-derived LH standards. No significant differences were found among the slopes of the curves elicited by the pituitary and recombinant FSH preparations. Each LH preparation exhibited a high degree of charge heterogeneity. Considerable variations in charge isoform distribution among the WHO LH standards, rhLH and LER-907 were also evident. In contrast, the FSH preparations were less heterogeneous and exhibited minor differences in charge distribution. Despite the existing differences in charge isoform distribution, all the pituitary-derived preparations as well as rhFSH seem appropriate for using as calibration standards in this particular EIA system.