Benzaldehyde dehydrogenase from chitosan-treated Sorbus aucuparia cell cultures

• Published in: Journal of plant physiology Vol. 166; no. 13; pp. 1343 - 1349
• Main Authors: Gaid, Mariam M., Sircar, Debabrata, Beuerle, Till, Mitra, Adinpunya, Beerhues, Ludger
• Format: Journal Article
• Language: English
• Published: Munich Elsevier GmbH 01.09.2009; Elsevier
• Subjects: acyltransferases; aldehyde oxidoreductases; Aldehyde Oxidoreductases - metabolism; aucuparin; benzaldehyde; Benzaldehyde dehydrogenase; benzaldehyde dehydrogenase (NAD+); Benzaldehydes - chemistry; Benzoic acid biosynthesis; Biocatalysis - drug effects; Biological and medical sciences; Biphenyl metabolism; biphenyl synthase; Cells, Cultured; chitosan; Chitosan - pharmacology; Chromatography, High Pressure Liquid; disease resistance; enzyme activity; enzyme substrates; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Kinetics; Multienzyme Complexes - metabolism; NAD (coenzyme); Phytoalexin; phytoalexins; Plant physiology and development; resistance mechanisms; Sorbus - cytology; Sorbus - drug effects; Sorbus - enzymology; Sorbus aucuparia; substrate specificity; Substrate Specificity - drug effects; BD; Biphenyl metabolism; DTT; Phytoalexin; Benzaldehyde dehydrogenase; Sorbus aucuparia; Benzoic acid biosynthesis; BIS; MSTFA; Benzoic acid; Cell culture; Enzyme; Rosaceae; Broadleaved ornamental tree; Biosynthesis; Dehydrogenase; Metabolism; Plant physiology; Dicotyledones; Angiospermae; Biphenyl; Spermatophyta; Oxidoreductases; Chitosan; Phytoatexin
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2009.03.003

Cell cultures of Sorbus aucuparia respond to the addition of chitosan with the accumulation of the biphenyl phytoalexin aucuparin. The carbon skeleton of this inducible defense compound is formed by biphenyl synthase (BIS) from benzoyl-CoA and three molecules of malonyl-CoA. The formation of benzoyl-CoA proceeds via benzaldehyde as an intermediate. Benzaldehyde dehydrogenase (BD), which converts benzaldehyde into benzoic acid, was detected in cell-free extracts from S. aucuparia cell cultures. BD and BIS were induced by chitosan treatment. The preferred substrate for BD was benzaldehyde ( K m=49 μM). Cinnamaldehyde and various hydroxybenzaldehydes were relatively poor substrates. BD activity was strictly dependent on the presence of NAD + as a cofactor ( K m=67 μM).