Tunable cytotoxic aptamer–drug conjugates for the treatment of prostate cancer
Therapies that can eliminate both local and metastatic prostate tumor lesions while sparing normal organ tissue are desperately needed. With the goal of developing an improved drug-targeting strategy, we turned to a new class of targeted anticancer therapeutics: aptamers conjugated to highly toxic c...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 115; no. 18; pp. 4761 - 4766 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
01.05.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Therapies that can eliminate both local and metastatic prostate tumor lesions while sparing normal organ tissue are desperately needed. With the goal of developing an improved drug-targeting strategy, we turned to a new class of targeted anticancer therapeutics: aptamers conjugated to highly toxic chemotherapeutics. Cell selection for aptamers with prostate cancer specificity yielded the E3 aptamer, which internalizes into prostate cancer cells without targeting normal prostate cells. Chemical conjugation of E3 to the drugs monomethyl auristatin E (MMAE) and monomethyl auristatin F (MMAF) yields a potent cytotoxic agent that efficiently kills prostate cancer cells in vitro but does not affect normal prostate epithelial cells. Importantly, the E3 aptamer targets tumors in vivo and treatment with the MMAF-E3 conjugate significantly inhibits prostate cancer growth in mice, demonstrating the in vivo utility of aptamer–drug conjugates. Additionally, we report the use of antidotes to block E3 aptamer–drug conjugate cytotoxicity, providing a safety switch in the unexpected event of normal cell killing in vivo. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: B.P.G., M.L., and B.A.S. designed research; B.P.G., L.K., D.P.A., A.P.B., and C.K. performed research; B.P.G., L.K., D.P.A., A.P.B., and C.K. analyzed data; and B.P.G. wrote the paper. Edited by Ronald R. Breaker, Yale University, New Haven, CT, and approved March 26, 2018 (received for review October 9, 2017) 1Present address: Vitrisa Therapeutics, Durham, NC 27701. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.1717705115 |