Quantitative Analysis of the Rates for Repeat-Mediated Genome Instability in a Yeast Experimental System

Instability of repetitive DNA sequences causes numerous hereditary disorders in humans, the majority of which are associated with trinucleotide repeat expansions. Here, we describe a unique system to study instability of triplet repeats in a yeast experimental setting. Using fluctuation assay and th...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1672; p. 421
Main Authors Radchenko, Elina A, McGinty, Ryan J, Aksenova, Anna Y, Neil, Alexander J, Mirkin, Sergei M
Format Journal Article
LanguageEnglish
Published United States 01.01.2018
Subjects
Repeat expansions
FluCalc
Repeat-induced mutagenesis
Trinucleotide repeats
Fluctuation assay
Expansion rate
Mutation rate
MSS-MLE
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Summary:Instability of repetitive DNA sequences causes numerous hereditary disorders in humans, the majority of which are associated with trinucleotide repeat expansions. Here, we describe a unique system to study instability of triplet repeats in a yeast experimental setting. Using fluctuation assay and the novel program FluCalc we are able to accurately estimate the rates of large-scale expansions, as well as repeat-mediated mutagenesis and gross chromosomal rearrangements for different repeat sequences.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7306-4_29