Rapid analysis of small samples containing forskolin using monoclonal antibodies

• Published in: Planta medica Vol. 62; no. 2; p. 169
• Main Authors: Yanagihara, H, Sakata, R, Shoyama, Y, Murakami, H
• Format: Journal Article
• Language: English
• Published: Germany 01.04.1996
• Subjects: analytical methods; Animals; Antibodies, Monoclonal; anticorps monoclonal; anticuerpos monoclonales; chemical composition; Chromatography, High Pressure Liquid; clone; clones; coleus; Colforsin - analysis; Colforsin - isolation & purification; composicion quimica; composition chimique; diterpenoide; diterpenoids; diterpenos; drug plants; Enzyme-Linked Immunosorbent Assay - methods; farmacologia; Humans; Mice; monoclonal antibodies; multiplication vegetative; pharmacologie; pharmacology; Plant Development; Plant Extracts; Plant Roots; plantas medicinales; plante medicinale; Plants - chemistry; propagacion vegetativa; Sensitivity and Specificity; Serum Albumin; technique analytique; tecnicas analiticas; vegetative propagation
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-2006-957844

The effective range of the competitive ELISA test for detection of forskolin content in clonally propagated plant organs of Coleus forskohlii using monoclonal antibodies extends from 5ng to 5 micrograms. A correlation between the forskolin accumulation and the growth rate was investigated using the clonally propagated shoots. An increase of forskolin content was noted, beginning at week 6. Flowers, rachises, leaves, stems, tuberous roots, and roots were analyzed. Tuberous roots and the stem base contained higher amounts of forskolin than other organs. The forskolin content in the stem decreased gradually towards the top of the shoot.