Nanoparticle interactions with zinc and iron: Implications for toxicology and inflammation

• Published in: Toxicology and applied pharmacology Vol. 225; no. 1; pp. 80 - 89
• Main Authors: Wilson, Martin R., Foucaud, Laurent, Barlow, Peter G., Hutchison, Gary R., Sales, Jill, Simpson, Richard J., Stone, Vicki
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.11.2007; Elsevier
• Subjects: 60 APPLIED LIFE SCIENCES; AEROSOLS; Air Pollutants - toxicity; AIR POLLUTION; Animals; Apoptosis - drug effects; Biological and medical sciences; CARBON BLACK; CATTLE; Cell Line; Chlorides - toxicity; Cytoskeleton; Cytoskeleton - drug effects; Cytoskeleton - metabolism; DNA; Drug Synergism; ENZYME IMMUNOASSAY; Enzyme-Linked Immunosorbent Assay; Ferric Compounds - toxicity; Flow Cytometry; GLUTATHIONE; INFLAMMATION; Inflammation - chemically induced; Inflammation Mediators - metabolism; IRON; Iron chloride; IRON CHLORIDES; Macrophage; Macrophages; Medical sciences; Mice; Microscopy, Confocal; Nanoparticle; Nanoparticles - toxicity; Necrosis - metabolism; NEUTROPHILS; PEROXIDASES; Phagocytosis; Phagocytosis - drug effects; PIPERAZINES; PM 10; Reactive Oxygen Species - metabolism; RNA; SCANNING ELECTRON MICROSCOPY; Soot - toxicity; SUPEROXIDE DISMUTASE; Toxicology; Tumor Necrosis Factor-alpha - drug effects; Tumor Necrosis Factor-alpha - metabolism; Welding fumes; ZINC; Zinc chloride; ZINC CHLORIDES; Zinc Compounds - toxicity; RNA; uf-CB; Nanoparticle; PM 2.5; HRP; DEFRA; FBS; GSH; CB; DIC; PBS; Carbon black; Zinc chloride; Welding fumes; IL-1; PMA; DCF; PM 10; DAPI; SOD; IL-8; BAL; Iron chloride; PMN; DCFH-DA; TNF-α; AV; DNA; HEPES; ROS; PI; Cytoskeleton; SEM; HBSS; Phagocytosis; ELISA; Macrophage; Welding; Inflammation; Ultrafine particle; Coarse particle; Toxicology; Aerosols; PM10; Fumes
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1016/j.taap.2007.07.012

Particulate air pollution (PM 10) consists of a mixture of components, including nanoparticles and metals. Studies from our laboratory have demonstrated that transition metals can potentiate the ability of nanoparticles to induce lung inflammation and that the zinc content of PM 10 was largely responsible for their potential to induce inflammation. These results are also relevant to zinc-containing engineered nanoparticles. To investigate the potential of ZnCl 2 and FeCl 3 to interact with nanoparticle carbon black in cell-free and biological systems to generate ROS, express pro-inflammatory mediators and cytotoxic ability. ROS production was examined using DCFH-DA. J774 cells were treated for 4 h with 14 nm CB and/or ZnCl 2 before measuring TNF-α by ELISA. Cytoskeletal changes were investigated using confocal microscopy. Flow cytometry was used to examine apoptotic/necrotic cells and phagocytic ability. In a cell-free system the particles generated significant ROS, whereas ZnCl 2 did not. Treatment of cells with 100 μM ZnCl 2, but not FeCl 3, increased TNF-α. Treatment with 14 nm CB alone induced TNF-α, which was synergistically enhanced by ZnCl 2. No interactions were observed in cells treated with 14 nm CB and FeCl 3. Cytoskeletal changes were observed with increasing concentrations of ZnCl 2. These results were confirmed by flow cytometry indicating that ZnCl 2 induced markers of apoptosis and necrosis. The phagocytic ability of cells was also significantly decreased. Nanoparticle carbon black alone did not induce changes in apoptosis/necrosis or the phagocytosis activity of the cells. Despite an inability to induce ROS production, ZnCl 2 stimulated TNF-α production which was synergistically enhanced by 14 nm carbon black. The ability of zinc to induce morphological changes and cell death was not altered by nanoparticle treatment.