Isolation and molecular identification of Lactobacillaceae bacteria and Bifidobacterium from horse feces

• Published in: Journal of Equine Science Vol. 36; no. 1; pp. 39 - 43
• Main Authors: FUJIMOTO, Riko, KUCHIDA, Momoka, BAN-TOKUDA, Tomomi, MATSUI, Hiroki
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society of Equine Science 01.01.2025; 日本ウマ科学会; Japan Science and Technology Agency; The Japanese Society of Equine Science
• Subjects: Bacteria; Bifidobacterium; Digestive system; Feces; Gastrointestinal tract; Intestinal microflora; Intestine; Lactic acid; lactic acid producing bacteria; Lactobacillaceae; Large intestine; Microbiota; Microorganisms; Probiotics; Racehorses; Lactobacillaceae; Bifidobacterium; lactic acid producing bacteria; probiotics
• ISSN: 1340-3516; 1347-7501
• DOI: 10.1294/jes.36.39

The equine large intestine harbors a diverse array of symbiotic microorganisms. Disruptions in the gut microbiota can lead to various diseases in horses. Probiotics offer promising avenues for enhancing equine health and performance. However, commercial formulations lack robust scientific validation. This study aimed to isolate and identify Lactobacillaceae and Bifidobacterium spp. from horse feces to explore their potential as probiotics. Fecal samples from Thoroughbred horses were subjected to isolation procedures. Lactic acid-producing bacteria were isolated using specific media and identified. The results revealed the isolation of Lactobacillaceae strains, including Limosilactobacillus equigenerosi, Ligilactobacillus equi, Ligilactobacillus agilis, and a Bifidobacterium sp., Bifidobacterium pseudolongum. These findings contribute to the understanding of equine gut microbiota and offer insights into potential probiotic candidates.