Roles of RodZ and class A PBP1b in the assembly and regulation of the peripheral peptidoglycan elongasome in ovoid‐shaped cells of Streptococcus pneumoniae D39

• Published in: Molecular microbiology Vol. 118; no. 4; pp. 336 - 368
• Main Authors: Lamanna, Melissa M., Manzoor, Irfan, Joseph, Merrin, Ye, Ziyun A., Benedet, Mattia, Zanardi, Alessia, Ren, Zhongqing, Wang, Xindan, Massidda, Orietta, Tsui, Ho‐Ching T., Winkler, Malcolm E.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.10.2022; John Wiley and Sons Inc
• Subjects: Assembly; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Cell cycle; Cell Division - genetics; Cell Wall - metabolism; class A PBP function and regulation; Depletion; elongasome assembly; Elongation; Filaments; Genetic relationship; Mutation; Peptidoglycan - metabolism; peptidoglycan synthesis; Peptidoglycans; Proteins; Scaffolding; Streptococcus infections; Streptococcus pneumoniae; Streptococcus pneumoniae - genetics; Streptococcus pneumoniae - metabolism; synthetic‐viable genetic relationships; Transmembrane domains; elongasome assembly; synthetic-viable genetic relationships; class A PBP function and regulation; peptidoglycan synthesis
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/mmi.14969

RodZ of rod‐shaped bacteria functions to link MreB filaments to the Rod peptidoglycan (PG) synthase complex that moves circumferentially perpendicular to the long cell axis, creating hoop‐like sidewall PG. Ovoid‐shaped bacteria, such as Streptococcus pneumoniae (pneumococcus; Spn) that lack MreB, use a different modality for peripheral PG elongation that emanates from the midcell of dividing cells. Yet, S. pneumoniae encodes a RodZ homolog similar to RodZ in rod‐shaped bacteria. We show here that the helix‐turn‐helix and transmembrane domains of RodZ(Spn) are essential for growth at 37°C. ΔrodZ mutations are suppressed by Δpbp1a, mpgA(Y488D), and ΔkhpA mutations that suppress ΔmreC, but not ΔcozE. Consistent with a role in PG elongation, RodZ(Spn) co‐localizes with MreC and aPBP1a throughout the cell cycle and forms complexes and interacts with PG elongasome proteins and regulators. Depletion of RodZ(Spn) results in aberrantly shaped, non‐growing cells and mislocalization of elongasome proteins MreC, PBP2b, and RodA. Moreover, Tn‐seq reveals that RodZ(Spn), but not MreCD(Spn), displays a specific synthetic‐viable genetic relationship with aPBP1b, whose function is unknown. We conclude that RodZ(Spn) acts as a scaffolding protein required for elongasome assembly and function and that aPBP1b, like aPBP1a, plays a role in elongasome regulation and possibly peripheral PG synthesis. This paper establishes RodZ as an essential scaffolding protein required for the assembly and function of the elongasome that synthesizes peripheral peptidoglycan (pPG) in Streptococcus pneumoniae, which lacks an MreB homolog. (Top panel) The assembly hierarchy mediated by RodZ(Spn). (Bottom panel) This paper also reports synthetic‐viable, suppressor relationships between Class A aPBP1b and aPBP1a and components of the core pPG elongasome, suggestive of failsafe bypass mechanisms that restore function of the pPG elongasome and viability.