Genetic diversity of wild and cultivated Coffea canephora in northeastern DR Congo and the implications for conservation

• Published in: American journal of botany Vol. 108; no. 12; pp. 2425 - 2434
• Main Authors: Vanden Abeele, Samuel, Janssens, Steven B., Asimonyio Anio, Justin, Bawin, Yves, Depecker, Jonas, Kambale, Bienfait, Mwanga Mwanga, Ithé, Ebele, Tshimi, Ntore, Salvator, Stoffelen, Piet, Vandelook, Filip
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America, Inc 01.12.2021; John Wiley and Sons Inc
• Subjects: Breeding; Clustering; Coffea - genetics; Coffea canephora; Coffee; Collection; Composition; Congo Basin; Conservation; crop domestication; crop wild relatives; Democratic Republic of the Congo; Differentiation; ex situ conservation; Genetic diversity; Genetic Markers; Genetic resources; Genetic Variation; Genotypes; INERA Yangambi; Microsatellites; Population genetics; Populations; Robusta coffee; Rubiaceae; Shrubs; tropical rainforest; Democratic Republic of the Congo; Congo Basin; INERA Yangambi; crop domestication; tropical rainforest; crop wild relatives; ex situ conservation; Rubiaceae; Robusta coffee
• ISSN: 0002-9122; 1537-2197
• DOI: 10.1002/ajb2.1769

Premise Many cultivated coffee varieties descend from Coffea canephora, commonly known as Robusta coffee. The Congo Basin has a century‐long history of Robusta coffee cultivation and breeding, and is hypothesized to be the region of origin of many of the cultivated Robusta varieties. Since little is known about the genetic composition of C. canephora in this region, we assessed the genetic diversity of wild and cultivated C. canephora shrubs in the Democratic Republic of the Congo. Methods Using 18 microsatellite markers, we studied the genetic composition of wild and backyard‐grown C. canephora shrubs in the Tshopo and Ituri provinces and multiple accessions from the INERA Yangambi Coffee Collection. We assessed genetic clustering patterns, genetic diversity, and genetic differentiation between populations. Results Genetic differentiation was relatively strong between wild and cultivated C. canephora shrubs, and both gene pools harbored multiple unique alleles. Strong genetic differentiation was also observed between wild populations. The level of genetic diversity in wild populations was similar to that of the INERA Yangambi Coffee Collection, but local wild genotypes were mostly missing from that collection. Shrubs grown in the backyards were genetically similar to the breeding material from INERA Yangambi. Conclusions Most C. canephora that is grown in local backyards originated from INERA breeding programs, while a few shrubs were obtained directly from surrounding forests. The INERA Yangambi Coffee Collection could benefit from an enrichment with local wild genotypes to increase the genetic resources available for breeding purposes and to support ex situ conservation.