Peripheral nerve injury increases contribution of L-type calcium channels to synaptic transmission in spinal lamina II: Role of α2δ–1 subunits

• Published in: Molecular pain Vol. 14; p. 1744806918765806
• Main Authors: Alles, Sascha RA, Garcia, Esperanza, Balasubramanyan, Sridhar, Jones, Karen, Tyson, John R, Joy, Twinkle, Snutch, Terrance P, Smith, Peter A
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.01.2018; Sage Publications Ltd
• Subjects: Calcium channels; Calcium channels (L-type); Calcium channels (voltage-gated); Calcium influx; Dihydropyridine; Dorsal horn; Excitatory postsynaptic potentials; Gabapentin; Neurons; Neurotransmitter release; Oocytes; Peripheral nerves; Peripheral neuropathy; Sensory neurons; Synaptic transmission; Allodynia; neuropathic pain; gabapentin; substantia gelatinosa; calcium channel blocker; pharmacology; anti-allodynic; chronic constriction injury; central sensitization; dihydropyridine
• ISSN: 1744-8069; 1744-8069
• DOI: 10.1177/1744806918765806

Background Following peripheral nerve chronic constriction injury, the accumulation of the α2δ–1 auxiliary subunit of voltage-gated Ca2+ channels in primary afferent terminals contributes to the onset of neuropathic pain. Overexpression of α2δ–1 in Xenopus oocytes increases the opening properties of Cav1.2 L-type channels and allows Ca2+ influx at physiological membrane potentials. We therefore posited that L-type channels play a role in neurotransmitter release in the superficial dorsal horn in the chronic constriction injury model of neuropathic pain. Results Whole-cell recording from lamina II neurons from rats, subject to sciatic chronic constriction injury, showed that the L-type Ca2+ channel blocker, nitrendipine (2 µM) reduced the frequency of spontaneous excitatory postsynaptic currents. Nitrendipine had little or no effect on spontaneous excitatory postsynaptic current frequency in neurons from sham-operated animals. To determine whether α2δ–1 is involved in upregulating function of Cav1.2 L-type channels, we tested the effect of the α2δ–1 ligand, gabapentin (100 µM) on currents recorded from HEK293F cells expressing Cav1.2/β4/α2δ–1 channels and found a significant decrease in peak amplitude with no effect on control Cav1.2/β4/α2δ–3 expressing cells. In PC-12 cells, gabapentin also significantly reduced the endogenous dihydropyridine-sensitive calcium current. In lamina II, gabapentin reduced spontaneous excitatory postsynaptic current frequency in neurons from animals subject to chronic constriction injury but not in those from sham-operated animals. Intraperitoneal injection of 5 mg/kg nitrendipine increased paw withdrawal threshold in animals subject to chronic constriction injury. Conclusion We suggest that L-type channels show an increased contribution to synaptic transmission in lamina II dorsal horn following peripheral nerve injury. The effect of gabapentin on Cav1.2 via α2δ–1 may contribute to its anti-allodynic action.