Ultrasound‐assisted solubilization of calcium from micrometer‐scale ground fish bone particles

• Published in: Food science & nutrition Vol. 10; no. 3; pp. 712 - 722
• Main Authors: Guo, Juanjuan, Zhu, Siliang, Chen, Hongbin, Zheng, Zongping, Pang, Jie
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.03.2022; John Wiley and Sons Inc
• Subjects: Bones; Calcium; calcium release; Cavitation; Collagen; Crystals; Enzymes; Fibrils; Fish; fish bone particles; Fourier analysis; Fourier transforms; Hydrogen bonding; Hydroxyapatite; Infrared analysis; mineralized collagen fibrils; Original; Original Research; Particle size; Solubilization; Ultrasonic imaging; ultrasonication; Ultrasound; Vertebrae; mineralized collagen fibrils; ultrasonication; fish bone particles; calcium release
• ISSN: 2048-7177; 2048-7177
• DOI: 10.1002/fsn3.2696

In order to promote the extraction of biological calcium from fish bone, ultrasonication was used to process micrometer‐scale fish bone particles (MFPs) and investigate the mechanism of action in relation to bone structure. With ultrasonication treatment (300 W, 60°C, 2 h), the content of calcium release increased by 25.6%. Calcium release reached 94.0% of total calcium after 24‐h treatment. The surface of the MFPs was significantly damaged by ultrasound‐induced cavitation, resulting in holes and separation of the layered structure. X‐ray diffraction (XRD) and Fourier transform infrared (FT‐IR) analysis demonstrated that the crystalline structure of hydroxyapatite was disrupted, the triple helical structure of mineralized collagen fibrils (MCFs) was loosened, and hydrogen bonding in collagen decreased, facilitating the release of hydroxyapatite crystals. Thus, ultrasonication may be a practical alternative to nanomilling for industrial processing of waste fish bones to produce soluble calcium as an ingredient in calcium supplements and supplemented foods. Our work was to promote calcium release from micrometer‐scale fish bone particles (MFPs) by ultrasonication and investigate the mechanism of action in relation to bone structure. Ultrasonication at 300 W, 60°C and for 2 h increased calcium release by 25.6%, and calcium release reached 94% of total calcium after 24‐h treatment. Furthermore, the crystalline structure of hydroxyapatite was disrupted, the triple helical structure of mineralized collagen fibrils (MCFs) was loosened, and hydrogen bonding in collagen decreased, facilitating the release of hydroxyapatite crystals.