DNA‐assisted site‐selective protein modification

Protein modification is important for various types of biomedical research, including proteomics and therapeutics. Many methodologies for protein modification exist, but not all possess the required level of efficiency and site selectivity. This review focuses on the use of DNA to achieve the desire...

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Bibliographic Details
Published inBiopolymers Vol. 113; no. 3; pp. e23483 - n/a
Main Authors Keijzer, Jordi F., Albada, Bauke
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.03.2022
Wiley Subscription Services, Inc
Subjects
aptamers
bioconjugation chemistry
Catalysis
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA chemistry
DNA, Catalytic
DNAzymes
Medical research
protein conjugates
Protein Processing, Post-Translational
Proteins
Proteins - metabolism
Proteomics
Review
Selectivity
DNA chemistry
bioconjugation chemistry
DNAzymes
aptamers
protein conjugates
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Summary:Protein modification is important for various types of biomedical research, including proteomics and therapeutics. Many methodologies for protein modification exist, but not all possess the required level of efficiency and site selectivity. This review focuses on the use of DNA to achieve the desired conversions and levels of accuracy in protein modification by using DNA (i) as a template to help concentrate dilute reactants, (ii) as a guidance system to achieve selectivity by binding specific proteins, and (iii) even as catalytic entity or construct to enhance protein modification reactions.
Bibliography:Funding information
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ObjectType-Article-2
SourceType-Scholarly Journals-1
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content type line 23
ObjectType-Review-1
Funding information Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ISSN:0006-3525
1097-0282
DOI:10.1002/bip.23483