Successful 18‐h acellular extracorporeal perfusion and replantation of porcine limbs ‐ Histology versus nerve stimulation

• Published in: Transplant international Vol. 34; no. 2; pp. 365 - 375
• Main Authors: Kruit, Anne Sophie, Brouwers, Kaj, Midden, Dominique, Zegers, Her, Koers, Erik, Alfen, Nens, Hummelink, Stefan, Ulrich, Dietmar J. O.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.02.2021; John Wiley and Sons Inc
• Subjects: acellular perfusion; Cold storage; Cryopreservation; Damage; Edema; ex‐vivo perfusion; Histology; in‐vivo function; limb replantation; Limbs; Median nerve; Muscle contraction; muscle function; Muscles; Muscular function; neurostimulation; Original; Perfusion; Stimulation; Subgroups; Surgery; Swine; acellular perfusion; ex-vivo perfusion; limb replantation; in-vivo function; muscle function; neurostimulation
• ISSN: 0934-0874; 1432-2277
• DOI: 10.1111/tri.13802

Summary The current standard for composite tissue preservation is static cold storage (SCS) and is limited to 6 h until irreversible muscle damage occurs. Extracorporeal perfusion (ECP) is a promising technique for prolonged preservation, however, functional results have been scarcely researched. This article assessed neuromuscular function and compared results to histological alterations to predict muscle damage after ECP. Forelimbs of twelve Dutch landrace pigs were amputated and preserved by 4 h SCS at 4–6 °C (n = 6) or 18 h mid‐thermic ECP with University of Wisconsin solution (n = 6). Limbs were replanted and observed for 12 h. Sham surgery was performed on contralateral forelimbs (n = 12). Histology analysis scored four subgroups representing different alterations (higher score equals more damage). Muscle contraction after median nerve stimulation was comparable between ECP, SCS, and sham limbs (P = 0.193). Histology scores were higher in ECP limbs compared to SCS limbs (4.8 vs. 1.5, P = 0.013). This was mainly based on more oedema in these limbs. In‐vivo muscle contraction was well preserved after 18 h ECP compared to short SCS, although histology seemed inferior in this group. Histology, therefore, did not correlate to muscle function at 12 h after replantation. This leads to the question whether histology or neuromuscular function is the best predictor for transplant success.