Evolution of BRET Biosensors from Live Cell to Tissue-Scale In vivo Imaging

Development of bioluminescence resonance energy transfer (BRET) based genetic sensors for sensing biological functions such as protein-protein interactions (PPIs) in vivo has a special value in measuring such dynamic events at their native environment. Since its inception in the late nineties, BRET...

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Published inFrontiers in endocrinology (Lausanne) Vol. 4; p. 131
Main Authors De, Abhijit, Jasani, Akshi, Arora, Rohit, Gambhir, Sanjiv S.
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 23.09.2013
Subjects
bioluminescence resonance energy transfer
Cell-based assay
Endocrinology
fluorescent proteins
luciferase
optical imaging
protein-protein interactions
protein–protein interactions
luciferase
fluorescent proteins
bioluminescence resonance energy transfer
cell-based assay
optical imaging
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Summary:Development of bioluminescence resonance energy transfer (BRET) based genetic sensors for sensing biological functions such as protein-protein interactions (PPIs) in vivo has a special value in measuring such dynamic events at their native environment. Since its inception in the late nineties, BRET related research has gained significant momentum in terms of adding versatility to the assay format and wider applicability where it has been suitably used. Beyond the scope of quantitative measurement of PPIs and protein dimerization, molecular imaging applications based on BRET assays have broadened its scope for screening pharmacologically important compounds by in vivo imaging as well. In this mini-review we focus on an in-depth analysis of engineered BRET systems developed and their successful application to cell-based assays as well as in vivo non-invasive imaging in live subjects.
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This article was submitted to Molecular and Structural Endocrinology, a section of the journal Frontiers in Endocrinology.
Reviewed by: Brian Hudson, University of Glasgow, UK; Stuart Maudsley, National Institutes of Health, USA
Edited by: Milka Vrecl, University of Ljubljana, Slovenia
ISSN:1664-2392
1664-2392
DOI:10.3389/fendo.2013.00131