HoxB8 neutrophils replicate Fcγ receptor and integrin‐induced neutrophil signaling and functions

• Published in: Journal of leukocyte biology Vol. 105; no. 1; pp. 93 - 100
• Main Authors: Chu, Julia Y., McCormick, Barry, Mazelyte, Greta, Michael, Melina, Vermeren, Sonja
• Format: Journal Article
• Language: English
• Published: United States John Wiley and Sons Inc 01.01.2019
• Subjects: adhesion molecules; Animals; Antigen-Antibody Complex - metabolism; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; Brief Conclusive Report; Cell Degranulation; Cell Development, Differentiation, & Trafficking; Chemotaxis; Fc Receptors; granulocytes; Homeodomain Proteins - metabolism; human cell lines; Integrins - metabolism; intracellular signaling; Mice, Inbred C57BL; monocytes/macrophages; neutrophils; Neutrophils - cytology; Neutrophils - metabolism; Neutrophils - physiology; Reactive Oxygen Species - metabolism; Receptors, IgG - metabolism; Signal Transduction; transgenic/knockout mice; granulocytes; Fc Receptors; monocytes/macrophages; adhesion molecules; intracellular signaling; human cell lines; neutrophils; transgenic/knockout mice
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/JLB.1AB0618-232R

Neutrophils are short‐lived, terminally differentiated leukocytes that form an essential part of host immunity and play a key role in acute and chronic inflammation. The analysis of these important cells is hindered by the fact that neutrophils are not amenable to culture, transfection, or transduction. Conditionally HoxB8‐immortalized mouse hematopoietic progenitors are suitable for in vitro differentiation of a range of myeloid cells, including neutrophils. Integrins and FcγRs are cell surface receptors, the ligation of which is required for a range of neutrophil functions that are important in health and disease. We show here that HoxB8 neutrophils express major neutrophil integrins and FcγRs. They respond to FcγR and integrin stimulation in a manner that is comparable with primary neutrophils, in terms of intracellular signaling. HoxB8 neutrophils also perform a range of FcγR/integrin‐dependent neutrophil functions, including, generation of reactive oxygen species, degranulation, and chemotaxis. Our findings suggest that HoxB8 neutrophils represent a faithful experimental model system for the analysis of Fc and integrin receptor‐dependent neutrophil functions. HoxB8 neutrophils presents to be a reliable and useful system for the analysis of Fc and integrin receptor‐dependent neutrophil functions.