Well-defined sulfobetaine-based statistical copolymers as potential antibioadherent coatings

• Published in: Journal of biomedical materials research Vol. 52; no. 1; p. 88
• Main Authors: Lowe, A B, Vamvakaki, M, Wassall, M A, Wong, L, Billingham, N C, Armes, S P, Lloyd, A W
• Format: Journal Article
• Language: English
• Published: United States 01.10.2000
• Subjects: 3T3 Cells; Animals; Bacterial Adhesion - drug effects; Betaine - pharmacology; Biocompatible Materials; Humans; Macrophages; Methacrylates - pharmacology; Mice; Polymers - pharmacology; Pseudomonas aeruginosa
• ISSN: 0021-9304
• DOI: 10.1002/1097-4636(200010)52:1<88::aid-jbm11>3.0.co;2-#

The potential use of novel poly(sulfobetaine) copolymers as antibioadherent coatings was investigated using Pseudomonas aeruginosa as a model microorganism and human macrophages and 3T3 mouse embryonic fibroblasts. Two well-defined statistical copolymers with narrow molecular weight distributions were prepared by group transfer copolymerization of n-butyl methacrylate (nBuMA) with either 10 or 30 mol % 2-(dimethylamino)ethyl methacrylate (DMAEMA). Sulfobetainized nBuMA-DMAEMA copolymers (poly[sulfobetaine-stat-nBuMA]) were obtained by treating these precursor polymers with 1,3-propanesultone under mild conditions. Both proton NMR spectroscopy and elemental microanalyses indicated that essentially all the DMAEMA residues were derivatized in both copolymers. Poly(methyl methacrylate) (PMMA) discs were coated with the sulfobetainized nBuMA-DMAEMA copolymers and the bioadherent properties of these coated materials were compared with those of PMMA. Statistically significantly fewer (p<.05) bacteria, macrophages, and fibroblasts adhered to the poly(sulfobetaine-stat-nBuMA)-coated PMMA than to the uncoated PMMA. The poly(sulfobetaine-stat-nBuMA) copolymer containing the higher proportion (30 mol %) sulfobetainized DMAEMA residues proved to be the more effective antibioadherent coating. The antibioadherent properties of these coating materials may allow the cost-effective production of dirt-resistant, easy to clean work surfaces, bioinert coatings for medical devices, and antifouling coatings for marine, agricultural, and industrial applications.