Acute lymphoblastic leukemia clonal distribution between bone marrow and peripheral blood

Acute lymphoblastic leukemia (ALL) is often composed of numerous subclones. Here we test whether the clonal composition of the blood is representative of the bone marrow at leukemia onset. Using ultra‐deep IGH sequencing, we detected 28 clones across 16 patients; 5/28 were only in the marrow. In fou...

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Bibliographic Details
Published inPediatric blood & cancer Vol. 67; no. 6; pp. e28280 - n/a
Main Authors Fries, Carol, Adlowitz, Diana G., Spence, Janice M., Spence, John P., Rock, Philip J., Burack, W. Richard
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.06.2020
Subjects
Acute lymphoblastic leukemia
Bone composition
Bone marrow
clonal heterogeneity
Cloning
Heavy chains
Hematology
immunoglobulin heavy chain
Immunoglobulins
Leukemia
Lymphatic leukemia
next‐generation sequencing
Oncology
Pediatrics
Peripheral blood
Site selection
tissue site abundance
immunoglobulin heavy chain
acute lymphoblastic leukemia
clonal heterogeneity
next-generation sequencing
tissue site abundance
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Summary:Acute lymphoblastic leukemia (ALL) is often composed of numerous subclones. Here we test whether the clonal composition of the blood is representative of the bone marrow at leukemia onset. Using ultra‐deep IGH sequencing, we detected 28 clones across 16 patients; 5/28 were only in the marrow. In four patients, the most abundant clones differed between sites, including three in which the dominant medullary clones were minimally detectable in the blood. These findings demonstrate that the peripheral blood often underrepresents the genetic heterogeneity in a B‐ALL and highlight the potential impact of tissue site selection on the detection of minor subclones.
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ISSN:1545-5009
1545-5017
DOI:10.1002/pbc.28280