Protein-induced changes during the maturation process of human dendritic cells: A 2-D DIGE approach

• Published in: Proteomics. Clinical applications Vol. 2; no. 9; pp. 1349 - 1360
• Main Authors: Ferreira, Gabriela Bomfim, Overbergh, Lut, van Etten, Evelyne, Lage, Kasper, D'Hertog, Wannes, Hansen, Daniel Aaen, Maris, Michael, Moreau, Yves, Workman, Christopher T., Waelkens, Etienne, Mathieu, Chantal
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.09.2008; WILEY‐VCH Verlag; Wiley
• Subjects: 2-D DIGE; Biological and medical sciences; Dendritic cell; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genetics of the immune response; Immunobiology; Maturation; Network; Human; Dendritic cell; 2-D DICE; Maturation; Proteomics; Network; 2-D-DIGE electrophoresis; Cell differentiation; Protein
• ISSN: 1862-8346; 1862-8354
• DOI: 10.1002/prca.200800110

Dendritic cells (DCs) are unique antigen presenting cells, which upon maturation change from a specialized antigen‐capturing cell towards a professional antigen presenting cells. In this study, a 2‐D DIGE analysis of immature and mature DCs was performed, to identify proteins changing in expression upon maturation. The protein expression profile of immature and mature DCs, derived from CD14+ peripheral blood monocytes was investigated using two pH ranges (pH 4–7 and 6–9) (n = 4). Ninety one differentially expressed spots (p<0.01) were detected, from which we identified 74 spots (81.32%) corresponding to 41 different proteins. The proteins identified play a role in diverse processes, such as antigen processing/presentation, vesicle transport and cytoskeleton remodeling. In addition, a protein interaction network contained 29 (out of 41) proteins, suggesting that, although they functionally originate from distinct classes, these proteins are acting as a protein‐interactome. In conclusion, the proteins shown here to be altered in expression upon maturation are in line with the morphological and functional changes observed during the maturation process, providing a better understanding of the processes involved. This will open new avenues for investigating treatment regimens for immune‐associated disorders.