Protein-induced changes during the maturation process of human dendritic cells: A 2-D DIGE approach

Dendritic cells (DCs) are unique antigen presenting cells, which upon maturation change from a specialized antigen‐capturing cell towards a professional antigen presenting cells. In this study, a 2‐D DIGE analysis of immature and mature DCs was performed, to identify proteins changing in expression...

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Published inProteomics. Clinical applications Vol. 2; no. 9; pp. 1349 - 1360
Main Authors Ferreira, Gabriela Bomfim, Overbergh, Lut, van Etten, Evelyne, Lage, Kasper, D'Hertog, Wannes, Hansen, Daniel Aaen, Maris, Michael, Moreau, Yves, Workman, Christopher T., Waelkens, Etienne, Mathieu, Chantal
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.09.2008
WILEY‐VCH Verlag
Wiley
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Summary:Dendritic cells (DCs) are unique antigen presenting cells, which upon maturation change from a specialized antigen‐capturing cell towards a professional antigen presenting cells. In this study, a 2‐D DIGE analysis of immature and mature DCs was performed, to identify proteins changing in expression upon maturation. The protein expression profile of immature and mature DCs, derived from CD14+ peripheral blood monocytes was investigated using two pH ranges (pH 4–7 and 6–9) (n = 4). Ninety one differentially expressed spots (p<0.01) were detected, from which we identified 74 spots (81.32%) corresponding to 41 different proteins. The proteins identified play a role in diverse processes, such as antigen processing/presentation, vesicle transport and cytoskeleton remodeling. In addition, a protein interaction network contained 29 (out of 41) proteins, suggesting that, although they functionally originate from distinct classes, these proteins are acting as a protein‐interactome. In conclusion, the proteins shown here to be altered in expression upon maturation are in line with the morphological and functional changes observed during the maturation process, providing a better understanding of the processes involved. This will open new avenues for investigating treatment regimens for immune‐associated disorders.
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ark:/67375/WNG-7K2B1SHP-C
ArticleID:PRCA200800110
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1862-8346
1862-8354
DOI:10.1002/prca.200800110