Changes in crustacean hyperglycemic hormones in Pacific whiteleg shrimp Litopenaeus vannamei subjected to air-exposure and low-salinity stresses

• Published in: Fisheries science Vol. 78; no. 4; pp. 833 - 840
• Main Authors: Shinji, Junpei, Kang, Bong Jung, Okutsu, Tomoyuki, Banzai, Kota, Ohira, Tsuyoshi, Tsutsui, Naoaki, Wilder, Marcy N.
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 01.07.2012; Springer Nature B.V
• Subjects: Air exposure; Animals; Bioassays; Biomedical and Life Sciences; Chromatography; Crustaceans; Energy; Experiments; Fish & Wildlife Biology & Management; Fisheries; Fishery sciences; Food Science; Freshwater & Marine Ecology; Glucose; Hormones; Life Sciences; Liquid chromatography; Litopenaeus vannamei; Marine; Metabolism; Original Article; Peptides; Physiology; Salinity; Science; Sinuses; Sodium; Stress response; Studies; I, Pacific; Air exposure; Low salinity; Shrimp; Stress; Crustacean hyperglycemic hormone
• ISSN: 0919-9268; 1444-2906
• DOI: 10.1007/s12562-012-0514-4

Changes in crustacean hyperglycemic hormone (CHH)-family peptides in response to stress were investigated in Litopenaeus vannamei . Stress treatments consisted of air exposure and low salinity. High-performance liquid chromatography was used to quantify CHH-family peptides in the X-organ–sinus gland complex (XO–SG) in the eyestalks. Among the CHH-family peptides analyzed, only the level of sinus gland peptide-G (SGP-G) in the XO–SG was decreased. SGP-G was also detectable by Western blotting analysis in the hemolymph of animals subjected to stress. These results suggest that SGP-G was secreted from the XO–SG into the hemolymph during stress. Glucose levels in the hemolymph increased under conditions during which SGP-G was detected in the hemolymph. Hyperglycemia was also observed when SGP-G was injected. SGP-G may function to shift energy use to deal with stress.