Evidence of Horizontal Gene Transfer of 50S Ribosomal Genes rplB, rplD, and rplY in Neisseria gonorrhoeae
Horizontal gene transfer (HGT) in the penA and multidrug efflux pump genes has been shown to play a key role in the genesis of antimicrobial resistance in Neisseria gonorrhoeae . In this study, we evaluated if there was evidence of HGT in the genes coding for the ribosomal proteins in the Neisseria...
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Published in | Frontiers in microbiology Vol. 12; p. 683901 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
10.06.2021
|
Subjects | |
Online Access | Get full text |
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Summary: | Horizontal gene transfer (HGT) in the
penA
and multidrug efflux pump genes has been shown to play a key role in the genesis of antimicrobial resistance in
Neisseria gonorrhoeae
. In this study, we evaluated if there was evidence of HGT in the genes coding for the ribosomal proteins in the
Neisseria
genus. We did this in a collection of 11,659 isolates of
Neisseria
, including
N. gonorrhoeae
and commensal
Neisseria
species (
N. cinerea
,
N. elongata
,
N. flavescens
,
N. mucosa
,
N. polysaccharea
, and
N. subflava
). Comparative genomic analyses identified HGT events in three genes:
rplB
,
rplD
, and
rplY
coding for ribosomal proteins L2, L4 and L25, respectively. Recombination events were predicted in
N. gonorrhoeae
and
N. cinerea
,
N. subflava
, and
N. lactamica
were identified as likely progenitors. In total, 2,337, 2,355, and 1,127 isolates possessed L2, L4, and L25 HGT events. Strong associations were found between HGT in L2/L4 and the C2597T 23S rRNA mutation that confers reduced susceptibility to macrolides. Whilst previous studies have found evidence of HGT of entire genes coding for ribosomal proteins in other bacterial species, this is the first study to find evidence of HGT-mediated chimerization of ribosomal proteins. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Kentaro Miyazaki, National Institute of Advanced Industrial Science and Technology (AIST), Japan; Joseph P. Dillard, University of Wisconsin-Madison, United States Edited by: Miklos Fuzi, Semmelweis University, Hungary This article was submitted to Antimicrobials, Resistance and Chemotherapy, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.683901 |