Evaluation of the multiplex real-time PCR assays RealStar malaria S&T PCR kit 1.0 and FTD malaria differentiation for the differentiation of Plasmodium species in clinical samples

• Published in: Travel medicine and infectious disease Vol. 31; p. 101442
• Main Authors: Frickmann, Hagen, Wegner, Christine, Ruben, Stefanie, Behrens, Christoph, Kollenda, Hans, Hinz, Rebecca, Rojak, Sandra, Schwarz, Norbert G, Hagen, Ralf Matthias, Tannich, Egbert
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.09.2019; Elsevier Limited
• Subjects: Blood; Deoxyribonucleic acid; Differentiation; DNA; Erythrocytes; Human diseases; Humans; Infectious Disease; Infectious diseases; Laboratories; Malaria; Malaria - diagnosis; Malaria - parasitology; Microscopy; Molecular Typing - methods; Multiplex Polymerase Chain Reaction - standards; Non-endemic; Nucleotide sequence; Parasites; Pathogens; PCR; Plasmodium; Screening; Serial assessments; Species Specificity; Travel medicine; Tropical climate; Values; Vector-borne diseases; Germany; Screening; Serial assessments; Non-endemic; Malaria; Differentiation; PCR
• ISSN: 1477-8939; 1873-0442
• DOI: 10.1016/j.tmaid.2019.06.013

AbstractBackgroundTwo commercial PCR assays were assessed in a retrospective study to determine their reliability as tools for the differentiation of Plasmodium species in human blood. MethodsA total of 1022 blood samples from 817 patients with suspected or confirmed malaria submitted to the German National Reference Centre for Tropical Pathogens were subjected to malaria microscopy using thick and thin blood films as well as to a genus-specific malaria real-time PCR. Parasite-positive samples were analysed by RealStar Malaria S&T PCR Kit 1.0 (altona Diagnostics) and FTD Malaria Differentiation (Fast Track Diagnostics) multiplex real-time PCR assays targeting species-specific Plasmodium DNA. ResultsOut of the 1022 blood samples, 247 (24.2%) tested positive for Plasmodium spp. The two multiplex assays showed rather similar performance characteristics and provided concordant species information in 98.9% of samples positive by malaria microscopy and in 95.1% (RealStar) and 96.8% (FTD) of samples positive by genus-specific PCR. Compared to FTD, RealStar revealed slightly reduced sensitivity for submicroscopic, low-level P. falciparum infections, while FTD was unable to detect P. knowlesi. ConclusionsThe two commercial malaria PCR assays assessed are suitable for discriminating Plasmodium species in clinical samples, and can provide additional information in cases of microscopically uncertain findings.