Characterizations of myosin essential light chain’s N-terminal truncation mutant Δ43 in transgenic mouse papillary muscles by using tension transients in response to sinusoidal length alterations

• Published in: Journal of muscle research and cell motility Vol. 34; no. 2; pp. 93 - 105
• Main Authors: Wang, Li, Muthu, Priya, Szczesna-Cordary, Danuta, Kawai, Masataka
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2013
• Subjects: Adenosine Diphosphate - genetics; Adenosine Diphosphate - metabolism; Adenosine Triphosphate - genetics; Adenosine Triphosphate - metabolism; Animal Anatomy; Animals; Biomedical and Life Sciences; Biomedicine; Calcium; Cell Biology; Histology; Kinetics; Life Sciences; Mice; Mice, Transgenic; Morphology; Myocardial Contraction; Myosin Light Chains - genetics; Myosin Light Chains - metabolism; Original Paper; Papillary Muscles - metabolism; Papillary Muscles - pathology; Papillary Muscles - physiopathology; Phosphates - metabolism; Proteomics; Sequence Deletion; Rate constants; Cardiac muscle; Muscle mechanics; Cardiomyopathy; Sinusoidal analysis; Cross-bridge kinetics; Elementary steps; Cross-bridge cycle; Tension transients
• ISSN: 0142-4319; 1573-2657
• DOI: 10.1007/s10974-013-9337-x

Cross-bridge kinetics were studied at 20 °C in cardiac muscle strips from transgenic (Tg) mice expressing N-terminal 43 amino acid truncation mutation (Δ43) of myosin essential light chain (ELC), and the results were compared to those from Tg-wild type (WT) mice. Sinusoidal length changes were applied to activated skinned papillary muscle strips to induce tension transients, from which two exponential processes were deduced to characterize the cross-bridge kinetics. Their two rate constants were studied as functions of ATP, phosphate (Pi), ADP, and Ca 2+ concentrations to characterize elementary steps of the cross-bridge cycle consisting of six states. Our results demonstrate for the first time that the cross-bridge kinetics of Δ43 are accelerated owing to an acceleration of the rate constant k 2 of the cross-bridge detachment step, and that the number of strongly attached cross-bridges are decreased because of a reduction of the equilibrium constant K 4 of the force generation step. The isometric tension and stiffness of Δ43 are diminished compared to WT, but the force per cross-bridge is not changed. Stiffness measurement during rigor induction demonstrates a reduction in the stiffness in Δ43, indicating that the N-terminal extension of ELC forms an extra linkage between the myosin cross-bridge and actin. The tension - pCa study demonstrates that there is no Ca 2+ sensitivity change with Δ43, but the cooperativity is diminished. These results demonstrate the importance of the N-terminal extension of ELC in maintaining the myosin motor function during force generation and optimal cardiac performance.