Core Genome MLST for Source Attribution of Campylobacter coli
Campylobacter species are among the leading foodborne bacterial agents of human diarrheal illness. The majority of campylobacteriosis has been attributed to Campylobacter jejuni (85% or more), followed by Campylobacter coli (5–10%). The distribution of C. jejuni and C. coli varies by host organism,...
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Published in | Frontiers in microbiology Vol. 12; p. 703890 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
13.07.2021
|
Subjects | |
Online Access | Get full text |
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Summary: | Campylobacter
species are among the leading foodborne bacterial agents of human diarrheal illness. The majority of campylobacteriosis has been attributed to
Campylobacter jejuni
(85% or more), followed by
Campylobacter coli
(5–10%). The distribution of
C. jejuni
and
C. coli
varies by host organism, indicating that the contribution to human infection may differ between isolation sources. To address the relative contribution of each source to
C. coli
infections in humans, core genome multilocus sequence type with a 200-allele difference scheme (cgMLST
200
) was used to determine cgMLST type for 3,432
C. coli
isolated from food animals (
n
= 2,613), retail poultry meats (
n
= 389), human clinical settings (
n
= 285), and environmental sources (
n
= 145). Source attribution was determined by analyzing the core genome with a minimal multilocus distance methodology (MMD). Using MMD, a higher proportion of the clinical
C. coli
population was attributed to poultry (49.6%) and environmental (20.9%) sources than from cattle (9.8%) and swine (3.2%). Within the population of
C. coli
clinical isolates, 70% of the isolates that were attributed to non-cecal retail poultry, dairy cattle, beef cattle and environmental waters came from two cgMLST
200
groups from each source. The most common antibiotic resistance genes among all
C. coli
were
tetO
(65.6%),
bla
OXA
–
193
(54.2%),
aph(3′)-IIIa
(23.5%), and
aadE-Cc
(20.1%). Of the antibiotic resistance determinants, only one gene was isolated from a single source:
bla
OXA
–
61
was only isolated from retail poultry. Within cgMLST
200
groups, 17/17 cgMLST
200
-435 and 89/92 cgMLST
200
-707 isolates encoded for
aph(3’)-VIIa
and 16/16 cgMLST
200
-319 harbored
aph(2’)-If
genes. Distribution of
bla
OXA
alleles showed 49/50 cgMLST
200
-5 isolates contained
bla
OXA
–
498
while
bla
OXA
–
460
was present in 37/38 cgMLST
200
-650 isolates. The cgMLST
200
-514 group revealed both
ant
(6)
-Ia
and
sat4
resistance genes in 23/23 and 22/23 isolates, respectively. Also, cgMLST
200
-266 and cgMLST
200
-84 had GyrAT86I mutation with 16/16 (100%) and 14/15 (93.3%), respectively. These findings illustrate how cgMLST and MMD methods can be used to evaluate the relative contribution of known sources of
C. coli
to the human burden of campylobacteriosis and how cgMLST typing can be used as an indicator of antimicrobial resistance in
C. coli
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology Edited by: Xiaonan Lu, McGill University, Canada Reviewed by: Jasna Kovac, The Pennsylvania State University (PSU), United States; Linda Antionette Bester, University of KwaZulu-Natal, South Africa |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.703890 |