Influence of Different Dietary Fats on the Incorporation of Exogenous Fatty Acids into Rat Adipose Glycerides

• Published in: The Journal of nutrition Vol. 118; no. 12; pp. 1447 - 1454
• Main Authors: Lhuillery, Claude, Mebarki, Slimane, Lecourtier, Marie-José, Demarne, Yves
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.12.1988; American Society for Nutritional Sciences; American Society for Nutrition
• Subjects: adipose tissue; Adipose Tissue - analysis; Adipose Tissue - metabolism; Animals; Biological and medical sciences; dietary fat; Dietary Fats - pharmacology; fatty acid incorporation; Fatty Acids - analysis; Fatty Acids - metabolism; Feeding. Feeding behavior; Food and Nutrition; Fundamental and applied biological sciences. Psychology; Glycerides - analysis; Glycerides - metabolism; Life Sciences; Male; Rats; Rats, Inbred Strains; Vertebrates: anatomy and physiology, studies on body, several organs or systems; adipose tissue; dietary fat; fatty acid incorporation; Adipocyte; Vertebrata; Incorporation; Mammalia; Nutrition; Rat; Diet; Body weight; Rodentia; Lipids; Fatty acids; RAT
• ISSN: 0022-3166; 1541-6100
• DOI: 10.1093/jn/118.12.1447

Male Wistar rats were fed for 6 wk either a control low fat diet (1.5% sunflower seed oil) or a diet containing 10% fat: either saturated (coconut oil, cocoa butter) or unsaturated (olive oil, sunflower seed oil). In each dietary condition, in vitro incorporation of exogenously added fatty acids (ranging from capric to oleic acid) was studied in epididymal adipose glycerides. Analysis of variance of data revealed that there was a significant effect of the diet × substrate interaction. When results were expressed per cell lipid weight medium-chain fatty acids (capric and lauric) were esterified to a lesser extent than long-chain fatty acids regardless of the nature of dietary fat (saturated vs. unsaturated). The nature of dietary fat was found to have no effect on the incorporation of medium-chain fatty acids. Feeding saturated fats resulted in an increase of incorporation of long-chain fatty acids into adipose glycerides whereas feeding unsaturated fats did not modify fatty acid incorporation. Modifications of mean fat cell size by dietary fat could not account for all the observed variations.